Composition & corresponding method of using spores of bacillus subtilis to stimulate and/or enhance immune response in mammals
AssigneeKing's College London
InventorsLinda Sylvia Klaviniskis, Andrew Graham Colin Barnes
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Abstract
Disclosed is a composition for administration to a human or animal subject, the composition containing spores of Bacillus subtilis in an amount effective to stimulate immune responsiveness in the subject. The spores have an adjuvant, immunomodulatory, immune potentiation, or dendritic cell maturation effect, or a combination thereof Also disclosed is a method of boosting an immune response in an animal subject by administering B. subtilis spores to an animal or human subject. Further disclosed is a vaccine containing B. subtilis spores, in the presence or absence of an antigen.
Claims33
A pharmaceutical composition for administration to a human or animal subject, the composition comprising an amount of spores of Bacillus subtilis, the amount being effective to stimulate or enhance immune responsiveness in the subject.
A human or animal vaccine comprising an immune response-stimulatory amount of spores of Bacillus subtilis in a pharmaceutically acceptable carrier or diluent for local, systemic, or mucosal delivery.
A method of boosting an immune response in an animal or human subject, the method comprising administering to the animal or human subject an amount of spores of Bacillus subtilis, the amount being effective to stimulate or enhance immune responsiveness in the subject.
A method of maturing dendritic cells or antigen presenting cells, the method comprising treating the cells with a maturation-effective amount of spores of Bacillus subtilis.
A method for modulation of an adjuvant effect in an organism, said method comprising:
- a) generating a viable or non-viable spore, wherein said spore has an adjuvant effect;
- b) isolating said spore;
- and
- c) contacting said organism with said spore and a nucleic acid, peptide, or polypeptide.
A composition comprising a spore system, said spore system comprising a spore and at least one peptide, polypeptide, protein, carbohydrate, or nucleotide sequence having anti-pathogenic activity displayed on, bound to, or contained within said spore.
A method of enhancing an immune response to an immunogenic polypeptide or peptide in a subject, said method comprising administering to the subject a population of spores and an immunogenic polypeptide or peptide, wherein the immune response to the immunogenic polypeptide or peptide is enhanced compared to the immune response generated by administration of the immunogenic polypeptide or peptide alone to the subject.
The method of claim 36, wherein said polypeptide is selected from the group consisting of antigens, adjuvants, co-stimulatory agents, and immunomodulatory agents.
Dependencies are inferred from the claim text.