Parvovirus capsids
AssigneeThe United States of America, as represented by the Secretary, Department of Health and Human Services
InventorsNeal S. Young, Sachiko Kajigaya, Shimada Takashi
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Abstract
The present invention relates to a method of producing non-infections parvovirus capsids and to diagnostic assays and vaccines utilizing same. The invention further relates to recombinant baculoviruses encoding parvovirus structural proteins and host cells infected therewith. The invention also relates to a method of packaging and delivering genetic information utilizing the noninfectious capsids.
Claims31
A method of producing parvovirus capsids comprising the steps of:
- i) introducing into a host cell a recombinant DNA molecule comprising:
- a) an expression vector, and
- b) a DNA sequence encoding the structural proteins of a parvovirus, with the proviso that genes encoding nonstructural parvovirus proteins are not included in the DNA sequence;
- ii) culturing the cells under conditions such that said structural proteins are produced and self assemble to form the capsids;
- and
- iii) isolating the capsids.
A parvovirus antigen consisting essentially of a parvovirus capsid.
A parvovirus antigen consisting essentially of a parvovirus capsid of major structural proteins free of minor structural proteins.
A recombinant baculovirus comprising a DNA segment encoding a minor structural protein of a parvovirus.
A recombinant baculovirus comprising a DNA segment encoding a major structural protein of a parvovirus.
A method of producing parvovirus capsids comprising the steps of:
- i) infecting an insect cell with (a) a first recombinant baculovirus comprising a DNA segment encoding a minor structural parvovirus protein and (b) a second recombinant baculovirus comprising a DNA segment encoding a major structural parvovirus protein;
- ii) culturing said cells under conditions such that said minor structural protein and said major structural protein are produced and self assemble to form the capsids;
- and
- iii) isolating the capsids.
A method of producing a protein presenting capsid comprising the steps of:
- i) coinfecting an insect cell with (a) a first recombinant baculovirus encoding a major structural parvovirus protein and (b) a second recombinant baculovirus encoding the nonunique region of a minor structural parvovirus protein and a nonparvovirus protein;
- ii) culturing said cells under conditions such that said expressed proteins self assemble to form the capsids;
- and
- iii) isolating the capsids.
A protein presenting capsid comprising a major structural parvovirus protein and a nonunique region of a minor structural parvovirus protein joined to a nonparvovirus protein.
Dependencies are inferred from the claim text.