Antibodies specific for seroreactive regions on HPV 16 proteins E1 and E2
AssigneeDade Behring Marburg GmbH
InventorsMartin Muller, Lutz Gissmann
Life of this patent
Estimating the term…
Abstract
The invention relates to seroreactive regions on proteins E1 and E2 of human papillomavirus (HPV) 16. The application also relates to a vaccine which contains such peptides which contain the seroreactive regions. The invention likewise embraces compositions for diagnostic purposes which contain peptides with the seroreactive regions.
Claims27
Seroreactive regions on the E1 protein of human papillomavirus (HPV) 16, with the following amino-acid sequences:
- 4 I. NGWFYVEAVVEXKTGDAISDDENENDSDTGEDLVDFIVNDNDYLT II. NENDSDTGEDLVDFIVND III. NADPAGTNGEEGTGCNGWFYVEAVVEKKTGDAISDPENENDSDTGED LVDFIVNDNDYLT IV. EDLVDFIVNDNDYLT V. EDLVDFIVNDNDYLTQAETETAHALFTAQEKQH VI. NENDSDTGEDLVDFIVNDNDYLTQAETETAHALFTAQEAXQHRDAVQVL KRKYL VII. GSPLSDIS.
Seroreactive regions on the E2 protein of human papillomavirus (HPV) 16, with the following amino-acid sequences:
- 5 I. DKILTHYENDS II. DKILTHYENDSTDLRDHI III. DLRDHIDYWKH IV. AIYYKAREMGFKHINHQVVPTLA V. AIYYKAREMGFKHINHQVVPTLAVSKNKAL VI. YYKAREMGFKHINHQVVPTLAVSKN VII. INHQVVPTLAVSKNKALQAI VIII. INHQVVPTLAVSKNKAL IX. TLAVSKNKALQAIELQLTLETIYNSQYSNEKWTLQDV X. QLTLETIYNSQYSNEKWTLQDVSLE XI. TLETIYNSQYSNEK XII. TSVFSSNEVSSPEII XIII. VFSSNEVSSPEIIRQHLANHPAATHTKAVALGTEET XIV. EIIRQHLANHPAATHTKAVALGTEETQTTIQRPRSEP
An antibody that binds to an amino acid sequence present in SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, SEQ ID NO:
- 9, SEQ ID NO:
- 10, SEQ ID NO:
- 11, SEQ ID NO:
- 12, SEQ ID NO:
- 13, SEQ ID NO:
- 14, SEQ ID NO:
- 16, SEQ ID NO:
- 17, SEQ ID NO:
- 18, SEQ ID NO:
- 19, SEQ ID NO:
- 20, SEQ ID NO:
- 21, or SEQ ID NO:
- 22.
A method of identifying, diagnosing, or detecting HPV 16 infection, said method comprising
- contacting a sample from a human suspected of being infected with HPV 16 with an antibody that binds to an amino acid sequence present in SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, SEQ ID NO:
- 7, SEQ ID NO:
- 8, SEQ ID NO:
- 9, SEQ ID NO:
- 10, SEQ ID NO:
- 11, SEQ ID NO:
- 12, SEQ ID NO:
- 13, SEQ ID NO:
- 14, SEQ ID NO:
- 16, SEQ ID NO:
- 17, SEQ ID NO:
- 18, SEQ ID NO:
- 19, SEQ ID NO:
- 20, SEQ ID NO:
- 21, or SEQ ID NO:
- 22, and
- detecting binding of said antibody to said amino acid sequence,
- wherein binding indicates an HPV 16 infection.
Dependencies are inferred from the claim text.