Methods of utilizing cultured hematopoietic progenitor cells for inducing immunological tolerance
OwnerYair Reisnerinventor
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Abstract
A method of inducing tolerance to a transplant transplanted from a donor to a recipient is disclosed. The method comprises (a) culturing a hematopoietic progenitor cell (HPC) population under growth conditions suitable for inducing or enhancing veto activity in at least a portion of the HPC population, thereby generating a tolerance-inducing cell population; and (b) administering a dose of the tolerance-inducing cell population prior to, concomitantly with or following transplantation of the transplant, thereby inducing tolerance to the transplant in the recipient.
Claims45
A method of inducing tolerance to a transplant transplanted from a donor to a recipient, the method comprising:
- (a) culturing an HPC population under growth conditions suitable for inducing or enhancing veto activity in at least a portion of said HPC population, thereby generating a tolerance-inducing cell population;
- and
- (b) administering a dose of said tolerance-inducing cell population prior to, concomitantly with or following transplantation of the transplant thereby inducing tolerance to the transplant in the recipient.
A method of transplanting a transplant derived from a donor to a recipient, the method comprising:
- (a) administering to the recipient a dose of cultured HPCs, said cultured HPCs having enhanced veto activity as compared to non-cultured HPCs;
- and
- (b) transplanting the transplant to the recipient.
A method of predicting the veto activity of a population of cultured HPCs, the method comprising:
- (a) identifying cells displaying a characteristic associated with a myeloid phenotype in the population of cultured HPCs;
- and
- (b) determining within the population of cultured HPCs a ratio between cells displaying said characteristic associated with a myeloid phenotype and cells not displaying said characteristic associated with a myeloid phenotype.
A method of isolating cells possessing veto activity from a population of cultured HPCs, th e method comprising:
- (a) contacting the population of cultured HPCs with a composition-of-matter capable of specifically binding to a cell displaying a characteristic associated with a myeloid phenotype;
- and
- (b) isolating said cells specifically contacting said composition-of-matter.
A method of treating or preventing an autoimmune disease in a subject, the method comprising administering to the subject a therapeutically effective amount of HPCs displaying at least one antigenic determinant associated with the autoimmune disease to thereby at least partially prevent or alleviate the autoimmune disease in the subject.
A population of cells comprising HPCs displaying at least one antigenic determinant associated with an autoimmune disease.
Dependencies are inferred from the claim text.