Compositions and methods for reporting of protease activity within the secretory pathway

An expression cassette comprising a chimeric nucleic acid encoding a polypeptide comprising a first, a second and a third domain, wherein the first domain comprises a Golgi retention signal peptide or an endoplasmic reticulum (ER) retention signal peptide, the second domain comprises a protease cleavage site, and the third domain comprises a reporter molecule, and the protease cleavage site is between the Golgi retention signal peptide and the reporter molecule.

An expression vector comprising a chimeric nucleic acid encoding a polypeptide comprising a first, a second and a third domain, wherein the first domain comprises a Golgi retention signal peptide or an endoplasmic reticulum (ER) retention signal peptide, the second domain comprises a protease cleavage site, and the third domain comprises a reporter molecule, and the protease cleavage site is between the Golgi retention signal peptide and the reporter molecule.

A transformed host cell comprising a nucleic acid encoding a chimeric polypeptide comprising a first, a second and a third domain, wherein the first domain comprises a Golgi retention signal peptide or an endoplasmic reticulum (ER) retention signal peptide, the second domain comprises a protease cleavage site, and the third domain comprises a reporter molecule, and the protease cleavage site is between the Golgi retention signal peptide and the reporter molecule.

A non-human transgenic animal comprising a nucleic acid encoding a chimeric polypeptide comprising a first, a second and a third domain, wherein the first domain comprises a Golgi retention signal peptide or an endoplasmic reticulum (ER) retention signal peptide, the second domain comprises a protease cleavage site, and the third domain comprises a reporter molecule, and the protease cleavage site is between the Golgi retention signal peptide and the reporter molecule.

A non-human transgenic animal that expresses a chimeric polypeptide comprising a first, a second and a third domain, wherein the first domain comprises a Golgi retention signal peptide or an endoplasmic reticulum (ER) retention signal peptide, the second domain comprises a protease cleavage site, and the third domain comprises a reporter molecule, and the protease cleavage site is between the Golgi retention signal peptide and the reporter molecule

A chimeric polypeptide comprising a first, a second and a third domain, wherein the first domain comprises a Golgi retention signal peptide or an endoplasmic reticulum (ER) retention signal peptide, the second domain comprises a protease cleavage site, and the third domain comprises a reporter molecule, and the protease cleavage site is between the Golgi retention signal peptide and the reporter molecule.

A chimeric polypeptide comprising a first, a second and a third domain, wherein the first domain comprises a Golgi retention signal peptide or an endoplasmic reticulum (ER) retention signal peptide, the second domain comprises a beta-secretase protease cleavage site, and the third domain comprises an alkaline phosphatase or a green fluorescent protein (GFP) reporter molecule, and the protease cleavage site is between the Golgi retention signal peptide and the reporter molecule.