Methods and compositions for chromatography
AssigneeKinetic Biosystems, Inc.
InventorHeath H. Herman
Life of this patent
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Abstract
The present invention is directed to methods and compositions for separating and isolating target molecules. In particular, the present invention comprises devices, such as CCDs, that contain particles without the need for support structures. Chromatography separation techniques, including but not limited to, ion exchange, size separation, affinity chromatography, ion exclusion, ligand exchange, reversed phase and normal phase partitioning, are used in the CCD. Methods also include low, medium and high pressure liquid chromatography. Such methods can be used for analytical, semi-preparative processes, initial clarification, preparative filtration and process scale applications.
Claims18
A method for isolating a target molecule, comprising,
- a) suspending chromatography particles in at least one chamber in a centrifugal force field wherein a continuous flow of a liquid acts to create a force which opposes the centrifugal force field and wherein a gravitational force contributes to the resultant vector summation of all forces acting on the particles, wherein the forces substantially immobilize the particles by the summation of the vector forces acting on the particles, and forming a chromatography bed,
- b) adding a heterogeneous liquid comprising the target molecule,
- c) separating the heterogeneous liquid by the actions of the chromatography bed;
- and
- d) retaining the separated portion of the heterogeneous liquid comprising the target molecule.
A method for performing chromatography, comprising,
- a) containing chromatography particles in at least one chamber in a centrifugal force field, the chamber having an inlet and an outlet;
- b) introducing a heterogeneous liquid to the chamber through the inlet in a liquid stream, wherein a continuous flow of a liquid acts to create a force which opposes the centrifugal force field and wherein a gravitational force contributes to the resultant vector summation of all forces acting on the particles, wherein the forces substantially immobilize the particles by the summation of the vector forces acting on the particles;
- c) collecting the liquid passing through the chamber and the particles,
- d) isolating a target molecule from either the liquid collected or from the chromatographic particles.
An apparatus for performing chromatography, comprising,
- at least one chamber with an inlet and outlet, the chamber adapted to,
- contain a chromatographic material,
- rotate the chamber to create a centrifugal force on the chromatographic material,
- receive a starting material through the inlet in a liquid stream, wherein a continuous flow of a liquid acts to create a force which opposes the centrifugal force field and wherein a gravitational force contributes to the resultant vector summation of all forces acting on the chromatographic material, wherein the forces substantially immobilize the chromatographic material by the summation of the vector forces acting on the chromatographic material.
Dependencies are inferred from the claim text.