Integrated microfluidic disc
AssigneeGyros AB
InventorsNigel Eric Tooke, Per Andersson
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Abstract
Disclosed is a method for performing the steps of nucleic acid template purification, a thermocycling reaction and purification of the products of the thermocycling reaction characterised in that the steps take place sequentially in a microfluidic disc. Also disclosed is a microstructure for fluids comprising at least one inlet opening connected to a first chamber incorporating a means for purifying template nucleic acid which, in turn, is connected to a second chamber incorporating a means for a thermocycling reaction which, in turn, is connected to a third chamber incorporating a means for purifying products of the thermocycling reaction, and a microfluidic disc comprising a plurality of such microstructures.
Claims12
A method for performing a sequence of steps comprising:
- a) the step of nucleic acid template purification;
- b) the step of a thermocycling reaction;
- and
- c) the step of purification of the products of step b)
- characterised in that the steps take place sequentially in a microfluidic disc.
A method for performing a nucleic acid sequencing reaction on a template nucleic acid, wherein the method comprises:
- a) treating a culture of cells containing a template nucleic acid with a lysis reagent so as to lyse the cytoplasmic membranes;
- b) introducing the lysate from step a) into microstructures for fluids on a microfluidic disc wherein each of said microstructures comprises a first chamber incorporating a means for purifying template nucleic acid, a second chamber incorporating a means for a thermocycling reaction and a third chamber incorporating a means for purifying products of the thermocycling reaction;
- and
- c) removing purified products for analysis.
A microstructure for fluids characterised in that it comprises:
- a) at least one inlet opening;
- connected to
- b) a first chamber incorporating a means for purifying template nucleic acid;
- connected to
- c) a second chamber incorporating a means for a thermocycling reaction;
- connected to
- d) a third chamber incorporating a means for purifying products of the thermocycling reaction.
A microstructure for fluids as claimed in claim further comprising:
- e) a fourth chamber incorporating a means for applying an electric potential across a separation matrix connected to the third chamber.
Dependencies are inferred from the claim text.