Buffers for electrophoresis and use thereof
AssigneeApplera Corporation
InventorsKevin J. Hacker, Karl O. Voss
Life of this patent
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Abstract
Various embodiments provide, for example, buffer compositions and/or sieving formulations useful in connection with electrophoresis instruments, such as capillary electrophoresis (CE) devices. In various embodiments, a buffer composition can include Bis-Tris, TAPS and/or TAPSO, and, optionally, a chelating agent, such as EDTA. Methods of separating samples containing bio-molecules, such as DNA or RNA, are also described.
Claims33
A buffer composition for electrophoresis of nucleic acids, the buffer composition comprising:
- (i) Bis-Tris;
- and
- (ii) one or more members of the group TAPS, TAPSO, and Asparagine.
A buffer composition for electrophoresis of nucleic acids, the buffer composition comprising:
- (i) TAPS, TAPSO, or Asparagine;
- and
- (ii) a compound of the formula:
- [HO(CH2)m]3C—N[(CH2)n—OH]2,
- wherein:
- m is an integer of from 1 to 3, and n is an integer of from 1 to 4.
A resolving-gel composition for electrophoresis of nucleic acids, the composition comprising:
- (a) an organic polymer,
- (b) Bis-Tris, and
- (c) one or more members of the group TAPS, TAPSO, and Asparagine.
An apparatus for resolving samples, comprising:
- an anodic buffer chamber;
- a cathodic buffer chamber;
- an electrophoretic channel extending between and communicating said anodic and cathodic buffer chambers;
- and
- a continuous buffer system comprising Bis-Tris held in said anodic and cathodic buffer chambers and in said channel.
A method of conducting electrophoresis of nucleic acids, comprising:
- adding a buffer into an electrophoretic channel, wherein said buffer comprises Bis-Tris;
- adding a sample including nucleic acids to be analyzed into said channel;
- and
- resolving said sample by electrophoresis.
A method of conducting electrophoresis of nucleic acids, comprising:
- (a) adding a buffer into an electrophoretic channel, wherein said buffer comprises a compound of the formula:
- [HO(CH2)m]3C—N[(CH2)n—OH]2,
- wherein m is an integer of from 1 to 3, and n is an integer of from 1 to 4;
- (b) adding a sample including nucleic acids to be analyzed into said channel;
- and
- (c) resolving said sample by electrophoresis.
A method for the electrophoresis of nucleic acids, comprising:
- introducing a buffer to a gel, wherein said buffer comprises Bis-Tris;
- applying a sample including nucleic acids to be analyzed on said gel;
- and
- applying an electromotive potential difference across said gel,
- whereby the sample is resolved.
Dependencies are inferred from the claim text.