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US 2003/0146091 A1Published application
Multiaperture sample positioning and analysis system
AssigneeMolecular Devices, LLC
InventorsHorst Vogel, Christian Schmidt
Life of this patent
Estimating the term…
Abstract
Systems for positioning and/or analyzing samples such as cells, vesicles, cellular organelles, and fragments, derivatives, and mixtures thereof, for electrical and/or optical analysis, especially relating to the presence and/or activity of ion channels.
Claims75
A system for analyzing membranous samples, comprising:
- a carrier with a shelf structure, the shelf structure having an aperture;
- first and second fluid compartments, separated by the carrier, and fluidically connected to one another, in the absence of a membranous sample, via the aperture;
- a membranous sample, selected from the group consisting of cells, organelles, and vesicles, sealed across the aperture, wherein the seal has an electrical resistance of greater than about 1 GΩ;
- and
- first and second electrodes, in electrical contact with the first and second fluid compartments, respectively, wherein the first and second electrodes are adapted to apply and/or measure at least one of an electrical potential and a current from at least a portion of the membranous sample sealed across the aperture.
2 more elements
A system for analyzing membranous samples, comprising:
- a carrier having an opening, the interior of the opening including a shelf structure;
- first and second fluid compartments, separated by the carrier, and fluidically connected to one another, in the absence of a membrane sample, via the opening;
- first and second electrodes, in electrical contact with the first and second fluid compartments, respectively, wherein the first and second electrodes being adapted to apply and/or measure at least one of an electrical potential and a current from at least a portion of a membranous sample sealed within the opening;
- and
- a membranous sample, selected from the group consisting of cells, organelles, and vesicles, sealing the opening.
2 more elements
A system for analyzing membranous samples, comprising:
- a carrier having a planar surface and an aperture terminating at the planar surface;
- first and second fluid compartments, separated by the carrier, and fluidically connected, in the absence of a sample, via the aperture;
- a membranous sample, selected from the group consisting of cells, organelles, and vesicles, sealed across the aperture, wherein the seal has an electrical resistance of greater than about 1 gigaohm;
- and
- first and second electrodes, in electrical contact with the first and second fluid compartments, respectively, wherein the first and second electrodes are adapted to apply and/or measure at least one of an electrical potential and a current from at least a portion of the membranous sample sealed across the aperture.
2 more elements
A system for analyzing membranous samples, comprising:
- a carrier comprising (i) a plurality of layers, the plurality including at least a first layer and a second layer having different compositions, and (ii) an aperture that traverses the first and second layers;
- first and second fluid compartments, separated by the carrier, and fluidically connected to one another, in the absence of a sample, via the aperture;
- a membranous sample, selected from the group consisting of cells, organelles, and vesicles, sealed across the aperture;
- and
- first and second electrodes, in electrical contact with the first and second fluid compartments, respectively, wherein the first and second electrodes are adapted to apply and/or measure at least one of an electrical potential and a current from at least a portion of the membranous sample sealed across the aperture.
2 more elements
A system for analyzing membranous samples, comprising:
- a carrier comprising (i) an aperture, and (ii) a binding surface associated with the aperture, the binding surface being adapted to bind a membranous sample such that the sample seals across the aperture;
- first and second fluid compartments, separated by the carrier, and fluidically connected to one another, in the absence of a sample, via the aperture;
- a membranous sample, selected from the group consisting of cells, organelles, and vesicles, bound to the binding surface, and sealed across the aperture;
- and
- first and second electrodes, in electrical contact with the first and second fluid compartments, respectively, wherein the first and second electrodes are adapted to apply and/or measure at least one of an electrical potential and a current from at least a portion of the membranous sample sealed across the aperture.
2 more elements
Dependencies are inferred from the claim text.