Methods of commercial production and extraction of protein from seed
AssigneeProdi Gene, Inc.
InventorJohn A. Howard
Life of this patent
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Abstract
A method for extraction of heterologous protein from plant seed comprises extracting the germ portion of the seed and extracting and purifying the protein from the germ. Enhanced expression in the germ is provided, and allows for improved efficiency in production, and cost savings. Directing expression to the germ portion further increases expression levels of the protein. The ubiquitin promoter preferentially directs expression to the germ portion of plant seed.
Claims21
A method of commercial production of heterologous proteins in plant tissue comprising:
- (a) transforming plant tissue which can produce seed having a germ capable of degermination in a commercial milling process, with a nucleotide sequence expressing the protein;
- (b) culturing a plant from the plant tissue;
- (c) growing the plant and harvesting the seed;
- (d) degerminating the seed in a commercial milling process such that the germ plant tissue has increased concentration of protein on a dry weight basis.
A method of decreasing the amount of plant tissue necessary to supply commercial quantities of heterologous proteins produced in the plant comprising transforming plant tissue which can produce seed having germ capable of degermination in a commercial milling process with a nucleotide sequence encoding the protein, growing the plant tissue such that it produces seed, separating the germ tissue of the seed and using the germ tissue to supply commercial quantities of the protein.
A method of supplying heterologous proteins produced in plants in a pharmaceutical, veterinary, agricultural or industrial use comprising transforming plant tissue which can produce seed having germ capable of degermination in a commercial milling process, growing the plant tissue such that it produces seed, separating the germ tissue of the plant and supplying the germ as the source of heterologous protein for said use.
A method of supplying heterologous protein expressed in plants for commercial applications comprising transforming a plant with a nucleotide sequence encoding the proteins, growing the plant, separating the germ tissue of the seed of the plant and supplying the germ as source of the protein in commercial applications.
A method of improving recovery of costs of production of heterologous proteins in plants comprising transforming a plant with a nucleotide sequence encoding the protein, growing the plant, separating the germ tissue of the seed of the plant, using the germ in commercial applications and using the remaining tissue of the seed in a second commercial application.
A method of selecting a promoter that preferentially directs expression of a linked sequence to the germ of plant seed comprising selecting from a group of promoters that includes the ubiquitin promoter.
Dependencies are inferred from the claim text.