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AssigneeProdiGene, Inc.
InventorsElizabeth Hood, John Howard, Donna Delaney
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Increased expression of heterologous proteins in a plant is achieved by introducing the nucleotide sequence encoding the heterologous protein into a plant that has high oil seed content compared to low oil seed and/or is introduced into a plant having reduced alcohol soluble proteins in the endosperm. The nucleotide sequence may be introduced by direct transformation into the plant, or by direct transformation into another plant, and crossing with the high oil plant or plant have reduced levels of alcohol soluble protein in the endosperm. Further, the nucleotide sequences may be introduced into one or both of the high oil plant and the plant having reduced levels of alcohol soluble proteins, and the plants may be crossed to result in a progeny having even further increased expression levels of the heterologous protein.
A method of increasing expression of a heterologous protein in the seed of a plant, the protein encoded by a recombinant nucleotide sequence, comprising introducing the nucleotide sequence into a plant comprising endosperm having reduced levels of alcohol soluble proteins in the endosperm.
A method of increasing expression of a heterologous protein in plant seed comprising introducing a recombinant nucleotide sequence encoding the protein into a plant which produces elevated levels of oil in seed on a percent dry weight basis when compared to low oil seed so that the ratio of heterologous protein to total soluble protein in seed of the plant increases.
The method above wherein the third plant is an opaque-2 plant.
A method of increasing expression of a heterologous protein in plant seed comprising introducing a nucleotide sequence encoding the protein into one of a high oil plant having increased oil content as compared to low oil plant varieties, or a plant having reduced levels of alcohol soluble proteins in the endosperm, to produce a first plant;
A method of selecting a plant host for production of increased levels of heterologous protein comprising:
A method of selecting a plant host for production of increased expression of heterologous protein comprising:
Dependencies are inferred from the claim text.