Isolation and identification of mouse and human transcription control elements associated with cytochrome expression
AssigneeXenogen Corporation
InventorsWeisheng Zhang, Pamela Contag, Anthony Purchio, Sandy Hashima, Shirley Ma, Kevin Nawotka
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Abstract
The present invention relates to transcription control elements derived from mouse and human genes associated with cytochrome expression, e.g., Cyp3A11 and CYP3A4, respectively. Isolated polynucleotides, expression cassettes, vectors, recombinant cells, and transgenic animals, may comprise such transcription control elements as described herein.
Claims36
An expression cassette comprising a polynucleotide derived from the mouse Cyp3A11 gene, said polynucleotide having at least 95% identity to nucleotides 1-11,002 of SEQ ID NO:12, said polynucleotide operably linked to a coding sequence of interest, wherein the polynucleotide comprises at least one transcriptional control element.
An expression cassette comprising, a polynucleotide derived from the mouse Cyp3A11 gene, said polynucleotide comprising a polynucleotide having at least 95% identity to the sequence of SEQ ID NO:13 or fragments of at least about 100 contiguous nucleotides of SEQ ID NO:13, said polynucleotide or fragments thereof operably linked to a coding sequence of interest, wherein the polynucleotide or fragments thereof comprise at least one transcriptional control element.
A transgenic rodent comprising, an expression cassette comprising a polynucleotide derived from the human CYP3A4 gene, said polynucleotide having at least 95% or greater identity to nucleotides 1-13,032 of SEQ ID NO:14, wherein (i) said polynucleotide is operably linked to a coding sequence of interest, (ii) the polynucleotide comprises at least one transcriptional control element, and (iii) expression of said coding sequence of interest is induced in the liver of the living, transgenic rodent by dexamethasone or rifampicin.
Dependencies are inferred from the claim text.