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US 2003/0145340 A1Published application
Ballistic transformation of C. elegans
OwnerRalph Schnabelinventor
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Abstract
A ballistic method of introducing nucleic acid into a nematode worm is described which involves bombarding the nematode with a plurality of microprojectiles. Nematode worms transformed according to the method of the invention are also provided.
Claims17
A method of introducing a nucleic acid into a nematode worm that is C. elegans, which method comprises:
- bombarding the worm with a plurality of microprojectiles under conditions to permit the plurality of the microprojectiles to penetrate the worm, wherein the microprojectiles are coated with the nucleic acid and wherein the worm is not dehydrated.
A method for selecting a nematode worm that is C. elegans and that heritably transmits a nucleic acid to a subsequent generation, which method comprises:
- (a) bombarding said worm with a plurality of microprojectiles under conditions to permit the nucleic acid to be retained in the worm, wherein the microprojectiles are coated with the nucleic acid;
- (b) selecting a transformant worm;
- (c) allowing the transformant worm to produce larvae that develop into a progeny worm;
- and
- (d) determining whether the progeny worm comprises the nucleic acid.
2 more elements
Dependencies are inferred from the claim text.