Peptide production
AssigneePPL Therapeutics (Scotland) Limited
InventorsAnthony John Clark, Richard Lathe
Life of this patent
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Abstract
A method of producing a substance comprising a peptide, involves incorporating a DNA sequence coding for the peptide into a gene of a mammal (such as a sheep) coding for a milk whey protein in such a way that the DNA sequence is expressed in the mammary gland of the adult female mammal. The substance may be an (optionally modified) protein such as a blood coagulation factor. The DNA sequence is preferably inserted into the first exon of a gene coding for a whey protein such as beta-lactoglobulin. The substance will generally be recovered from milk of the female mammal, but may (for example if it is an enzyme) be used in situ.
Claims33
A method of producing a substance comprising a polypeptide, the method comprising incorporating a DNA sequence coding for the polypeptide into a gene of a female mammal coding for a milk whey protein in such a way that the DNA sequence is expressed in the mammary gland of the adult female mammal.
A method of producing a substance which is the reaction product of an enzyme, the method comprising incorporating a DNA sequence coding for the enzyme into a gene of a mammal coding for a milk whey protein in such a way that the DNA sequence is expressed in the mammary gland of the adult female mammal, whereafter it catalyses the formation of the reaction product from one or more substrates of the enzyme.
A method of producing a substance comprising a polypeptide substantially as herein described.
A genetic construct comprising a DNA sequence encoding a polypeptide incorporated into a gene of a mammal tht codes for a milk whey protein in such a way that the DNA sequence is expressible in the mammary gland of the adult female mammal.
Dependencies are inferred from the claim text.