Human serine protease
AssigneeZymoGenetics, Inc.
InventorDarrell C. Conklin
Life of this patent
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Abstract
Members of the serine protease family play a role in carefully controlled processes, such as blood coagulation, fibrinolysis, complement activation, fertilization, and hormone production. These enzymes are also used in a variety of diagnostic, therapeutic, and industrial contexts. Ztryp3 is a new member of the serine protease family.
Claims27
An isolated polypeptide, comprising an amino acid sequence selected from the group consisting of:
- amino acid residues 20-to 235 of SEQ ID NO:2, amino acid residues 20 to 130 of SEQ ID NO:2, amino acid residues 20 to 198 of SEQ ID NO:2, and amino acid residues 40 to 198 of SEQ ID NO:2.
An isolated nucleic acid molecule, wherein the nucleic acid molecule is a nucleic acid molecule that remains hybridized following stringent wash conditions to a nucleic acid molecule selected from the group consisting of:
- (a) a nucleic acid molecule consisting of the nucleotide sequence of nucleotides 42 to 746 of SEQ ID NO:1, (b) a nucleic acid molecule consisting of the nucleotide sequence of nucleotides 99 to 746 of SEQ ID NO:1, and (c) a nucleic acid consisting of a nucleotide sequence that is a complement of the nucleotide sequence of nucleic acid molecule (a) or (b).
An isolated nucleic acid molecule that encodes a polypeptide comprising an amino acid sequence selected from the group consisting of:
- amino acid residues 20 to 235 of SEQ ID NO:2, amino acid residues 20 to 130 of SEQ ID NO:2, amino acid residues 20 to 198 of SEQ ID NO:2, and amino acid residues 40 to 198 of SEQ ID NO:2.
A method of detecting in a biological sample the presence of RNA that encodes the amino acid sequence of SEQ ID NO:2, comprising:
- (a) contacting a nucleic acid probe under hybridizing conditions with either (i) test RNA molecules isolated from the biological sample, or (ii) nucleic acid molecules synthesized from the isolated RNA molecules, wherein the probe has a nucleotide sequence comprising either a portion of the nucleotide sequence of nucleotides 42 to 746 of SEQ ID NO:1, or its complement, and
- (b) detecting the formation of hybrids of the nucleic acid probe and either the test RNA molecules or the synthesized nucleic acid molecules,
- wherein the presence of the hybrids indicates the presence of RNA that encodes the amino acid sequence of SEQ ID NO:2 in the biological sample.
An isolated polypeptide, comprising amino acid residues 20 to 235 of SEQ ID NO:2.
A composition, comprising a carrier and a polypeptide that comprises amino acid residues 20 to 235 of SEQ ID NO:2.
Dependencies are inferred from the claim text.