Anti-angiogenic proteins and fragments and methods of use thereof
AssigneeBeth Israel Deaconess Medical Center, Inc.
InventorRaghuram Kalluri
Life of this patent
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Abstract
Proteins with anti-angiogenic properties are disclosed, and fragments thereof, and methods of using those proteins and fragments to inhibit or promote angiogenesis.
Claims107
An isolated fragment of SEQ ID NO:10, having the ability to inhibit tumor growth.
An isolated mutated fragment of SEQ ID NO:10, wherein one or more, and five or fewer, amino acids have been substituted, and wherein the mutated fragment has the ability to inhibit tumor growth.
An isolated fragment of SEQ ID NO:10, having the ability to inhibit angiogenesis.
An isolated mutated fragment of SEQ ID NO:10, wherein one or more, and five or fewer, amino acids have been substituted, and wherein the mutated fragment has the ability to inhibit angiogenic activity.
An isolated fragment of SEQ ID NO:10, having the ability to inhibit protein synthesis in endothelial cells.
An isolated mutated fragment of SEQ ID NO:10, wherein one or more, and five or fewer, amino acids have been substituted, and wherein the mutated fragment has the ability to inhibit protein synthesis in endothelial cells.
A method for inhibiting tumor growth in mammalian tissue, the method comprising contacting the tissue with a composition comprising an isolated fragment selected from the group consisting of:
- (a) SEQ ID NO:10;
- (b) amino acid 2 through amino acid 245 of SEQ ID NO:10;
- (c) SEQ ID NO:19;
- (d) amino acid 1 through amino acid 125 of SEQ ID NO:10;
- (e) SEQ ID NO:20;
- (f) SEQ ID NO:21;
- (g) SEQ ID NO:22;
- (h) SEQ ID NO:23;
- (i) SEQ ID NO:25;
- (j) SEQ ID NO:26;
- (k) SEQ ID NO:29;
- (l) SEQ ID NO:30;
- (m) SEQ ID NO:33;
- (n) SEQ ID NO:34;
- (o) SEQ ID NO:37;
- (p) SEQ ID NO:38;
- (q) SEQ ID NO:39;
- (r) SEQ ID NO:40;
- (s) SEQ ID NO:41;
- and
- (t) SEQ ID NO:42.
A method for inhibiting angiogenic activity in mammalian tissue, the method comprising contacting the tissue with a composition comprising an isolated fragment selected from the group consisting of:
- (a) SEQ ID NO:10;
- (b) amino acid 2 through amino acid 245 of SEQ ID NO:10;
- (c) SEQ ID NO:19;
- (d) amino acid 1 through amino acid 125 of SEQ ID NO:10;
- (e) SEQ ID NO:20;
- (f) SEQ ID NO:21;
- (g) SEQ ID NO:22;
- (h) SEQ ID NO:23;
- (i) SEQ ID NO:25;
- (j) SEQ ID NO:26;
- (k) SEQ ID NO:29;
- (l) SEQ ID NO:30;
- (m) SEQ ID NO:33;
- (n) SEQ ID NO:34;
- (o) SEQ ID NO:37;
- (p) SEQ ID NO:38;
- (q) SEQ ID NO:39;
- (r) SEQ ID NO:40;
- (s) SEQ ID NO:41;
- and
- (t) SEQ ID NO:42.
A method for inhibiting protein synthesis in one or more mammalian cells, the method comprising contacting the one or more cells with a composition comprising an isolated fragment selected from the group consisting of:
- (a) SEQ ID NO:10;
- (b) amino acid 2 through amino acid 245 of SEQ ID NO:10;
- (c) SEQ ID NO:19;
- (d) amino acid 1 through amino acid 125 of SEQ ID NO:10;
- (e) SEQ ID NO:20;
- (f) SEQ ID NO:21;
- (g) SEQ ID NO:22;
- (h) SEQ ID NO:23;
- (i) SEQ ID NO:25;
- (j) SEQ ID NO:26;
- (k) SEQ ID NO:29;
- (l) SEQ ID NO:30;
- (m) SEQ ID NO:33;
- (n) SEQ ID NO:34;
- (o) SEQ ID NO:37;
- (p) SEQ ID NO:38;
- (q) SEQ ID NO:39;
- (r) SEQ ID NO:40;
- (s) SEQ ID NO:41;
- and
- (t) SEQ ID NO:42.
A method for inhibiting protein synthesis in one or more mammalian cells, the method comprising contacting the one or more cells with a composition comprising an isolated fragment selected from the group consisting of:
- (a) SEQ ID NO:2;
- (b) SEQ ID NO:6;
- and
- (c) SEQ ID NO:10.
An isolated peptide of the formula:
- R1X1LFX2NVNX3VX4NFR2 (SEQ ID NO:45),
- wherein R1 is hydrogen or a peptidyl chain of 1 to 17 amino acids, R2 is hydrogen or a peptidyl chain of 1 to 12 amino acids, and X1, X2 and X3 are individually an amino acid, and wherein said peptide inhibits tumor growth.
An isolated peptide of the formula:
- R1X1LFX2NVNX3VXNFR2 (SEQ ID NO:45),
- wherein R1 is hydrogen or a peptidyl chain of 1 to 17 amino acids, R2 is hydrogen or a peptidyl chain of 1 to 12 amino acids, and X1, X2 and X3 are individually an amino acid, and wherein said peptide inhibits angiogenic activity in mammalian tissue.
An isolated peptide of the formula:
- R1X1LFX2NVNX3VX4NFR2 (SEQ ID NO:45),
- wherein R1 is hydrogen or a peptidyl chain of 1 to 17 amino acids, R2 is hydrogen or a peptidyl chain of 1 to 12 amino acids, and X1, X2 and X3 are individually an amino acid, and wherein said peptide inhibits protein synthesis in endothelial cells.
Dependencies are inferred from the claim text.