Molecules designated B7L-1
AssigneeImmunex Corporation
InventorPeter R. Baum
Life of this patent
Estimating the term…
Abstract
The invention is directed to B7L-1 as a purified and isolated protein, the DNA encoding the B7L-1, host cells transfected with cDNAs encoding B7L-1 and processes for preparing B7L-1 polypeptides.
Claims15
An isolated DNA sequence encoding a polypeptide that is at least 80% identical to the sequence of amino acid residues selected from the group consisting of amino acids 1-432 of SEQ ID NO:2, amino acids 1-398 of SEQ ID NO:4, and amino acids 1-398 of SEQ ID NO:6, the polypeptide being capable of binding to a ldcam polypeptide:
An isolated DNA sequence encoding a polypeptide having an amino acid sequence selected from the group SEQ ID NO:2, SEQ ID NO:4, and SEQ ID NO:6.
An isolated DNA encoding a soluble polypeptide wherein said soluble polypeptide comprises an amino acid sequence that is at least 90% identical to a sequence selected from the group consisting of:
- a) amino acids x1 to 364 of SEQ ID NO:2, wherein x1 is amino acid 1 or 21;
- b) amino acids x1′ to 330 of SEQ ID NO:4, where x1′ is amino acid 1 or 21;
- c) amino acids y1 to 330 of SEQ ID NO:6, wherein y1 is amino acid 1 or 21;
- and
- d) a fragment of the sequences of a) b) or c),
- wherein the soluble polypeptide is capable of binding to a LDCAM polypeptide.
An isolated DNA encoding a soluble polypeptide wherein said soluble polypeptide comprises an amino acid sequence selected from the group consisting of:
- a) amino acids x1 to 364 of SEQ ID NO:2, wherein x1 is amino acid 1 or 21;
- b) amino acids x1′ to 330 of SEQ ID NO:4, where x1′ is amino acid 1 or 21;
- c) amino acids y1 to 330 of SEQ ID NO:6, wherein y1 is amino acid 1 or 21;
- and
- d) a fragment of the sequences of a) b) or c),
DNA selected from the group consisting of:
- a) nucleic acids x1 to 1452 of SEQ ID NO:1, wherein x1 is nucleic acid 157 or 217;
- b) nucleic acids x1′ to 1206 of SEQ ID NO:3, wherein x1′ is nucleic acid 13 or 73;
- c) nucleic acids y1 to 1350 of SEQ ID NO:5, wherein y1 is nucleic acid 157 or 217;
- d) DNA sequences that hybridize under moderately stringent conditions to the DNA of a) b) or c);
- and which DNA sequences encode a polypeptide that binds itself;
- and
- e) DNA complementary to the DNA of a), b) c) and d).
- f) DNA sequences that, due to the degeneracy of the genetic code, encode B7L-1 polypeptide having the amino acid sequence of the polypeptide encoded by the DNA sequences of a), b), c) d) or e).
A polypeptide encoded by DNA selected from the group consisting of:
- a) nucleic acids x1 to 1452 of SEQ ID NO:1, wherein x1 is nucleic acid 157 or 217;
- b) nucleic acids x1′ to 1206 of SEQ ID NO:3, wherein x1′ is nucleic acid 13 or 73;
- c) nucleic acids y1 to 1350 of SEQ ID NO:5, wherein y1 is nucleic acid 157 or 217;
- d) DNA sequences that hybridize under moderately stringent conditions to the DNA of a) b) or c);
- and which DNA sequences encode a polypeptide that binds itself;
- and
- e) DNA complementary to the DNA of a), b) c) and d).
- f) DNA sequences that, due to the degeneracy of the genetic code, encode B7L-1 polypeptide having the amino acid sequence of the polypeptide encoded by the DNA sequences of a), b), c) d) or e).
A polypeptide comprising an amino acid sequence that is at least 80% identical to an amino acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:4 and SEQ ID NO:6, the polypeptide being capable of binding to a LDCAM polypeptide.
A soluble polypeptide comprising an amino acid sequence selected from the group consisting of:
- a) amino acids x1 to 364 of SEQ ID NO:2, wherein x1 is amino acid 1 or 21;
- b) amino acids x1′ to 330 of SEQ ID NO:4, where x1′ is amino acid 1 or 21;
- c) amino acids y1 to 330 of SEQ ID NO:6, wherein y1 is amino acid 1 or 21;
- and
- d) a fragment of the sequences of a) b) or c),
- wherein the fragment is capable of binding to a LDCAM polypeptide.
A soluble polypeptide comprising an amino acid sequence that is at least 90% identical to an amino acids sequence selected from the group consisting of:
- a) amino acids x1 to 364 of SEQ ID NO:2, wherein x1 is amino acid 1 or 21;
- b) amino acids x1′ to 330 of SEQ ID NO:4, where x1′ is amino acid 1 or 21;
- c) amino acids y1 to 330 of SEQ ID NO:6, wherein y1 is amino acid 1 or 21;
- and
- d) a fragment of the sequences of a) b) or c)
- wherein the soluble polypeptide is capable of binding to a LDCAM polypeptide.
A fusion protein comprising an amino acid selected from the group consisting of:
- a) amino acids x1 to 364 of SEQ ID NO:2, wherein x1 is amino acid 1 or 21;
- b) amino acids x1′ to 330 of SEQ ID NO:4, where x1′ is amino acid 1 or 21;
- c) amino acids y1 to 330 of SEQ ID NO:6, wherein y1 is amino acid 1 or 21;
- and
- d) a fragment of the sequences of a) b) or c),
- wherein the fragment is capable of binding to a LDCAM polypeptide
Dependencies are inferred from the claim text.