T cell receptor fusions and conjugates and methods of use thereof
AssigneeSunol Molecular Corporation
InventorsJon A. Weidanz, Kimberlyn F. Card, Hing C. Wong
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Abstract
Featured is T cell receptor complexes designed to redirect the immune system against various diseases. The T cell receptor complexes of the invention have been engineered to recognize target antigen in a functionally bispecific nature. Fusion protein complexes and protein conjugate complexes are comprised of high affinity antigen-specific TCR and biologically active proteins and/or effector molecules. Also featured is methods of production of T cell receptor fusion and conjugate complexes as well as therapeutic compositions for use of the complexes.
Claims80
A soluble T cell receptor fusion complex comprising a T cell receptor and a biologically active polypeptide connected by a peptide linker, wherein the T cell receptor has one recognition binding site and the biologically active polypeptide has a different recognition binding site.
A method of preparing a soluble T cell receptor fusion complex, the method comprising:
- providing a T cell receptor chain, or subfragment thereof;
- providing a biologically active polypeptide corresponding to a second chain, or subfragment thereof;
- connecting the T cell receptor chain and the second chain to a peptide linker;
- and
- recovering the linked T cell receptor fusion polypeptide complex, thereby generating a T cell receptor fusion complex.
A soluble T cell receptor conjugate complex comprising a plurality of biologically active molecules covalently bound to a carrier having at least one remaining free amine group, the carrier being covalently bound to a portion of a single chain T cell receptor, wherein the resulting conjugate is soluble.
A method of preparing a soluble T cell receptor conjugate complex comprising:
- reacting a polymer carrier which has covalently bound a plurality of biologically active molecules and, with a T cell receptor chain;
- and
- reductively stabilizing the resulting conjugate molecule, wherein the resultant conjugate T cell receptor molecule is soluble.
A nucleic acid sequence encoding a T cell receptor fusion complex comprising a T cell receptor and a biologically active polypeptide connected by a peptide linker. wherein the T cell receptor has one recognition binding site and the biologically active polypeptide has a different recognition binding site.
Dependencies are inferred from the claim text.