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AssigneeSenesco Technologies, Inc.
InventorsJohn E. Thompson, Catherine Taylor, Dominic Cliche, Charles Dinarello, Leonid Reznikov, Benjamin Pomerantz
Estimating the term…
The present invention provides methods of identifying an incidence of ischemia in mammalian tissue, particularly mammalian heart tissue. Further, a method of reducing apoptosis in mammalian tissue, preferably heat tissue, is provided. These methods involve measuring and comparing the gene expression levels of both apoptosis-specific eIF-5A and proliferating eIF-5A and correlating an incidence of ischemia when the expression level of apoptosis-specific eIF-5a is higher than proliferating eIF-5A. In the method of reducing apoptosis in mammalian tissue, there is provided an agent that inhibits expression of apoptosis-specific eIF-5A. Preferred agents are antisense oligonucleotides to human apoptosis-specific eIF-5A.
An isolated nucleic acid comprising a nucleotide sequence encoding a rat apoptosis-specific eIF-5A polypeptide.
A purified polypeptide comprising an amino acid sequence of a rat apoptosis-specific eIF-5A polypeptide.
An isolated nucleic acid comprising a nucleotide sequence encoding a rat apoptosis-specific DHS polypeptide.
A purified polypeptide comprising an amino acid sequence of a rat apoptosis-specific DHS polypeptide.
A method of modulating apoptosis in a cell comprising administering to the cell an agent that modulates apoptosis-specific eIF-5A function.
A method of modulating apoptosis in a cell comprising administering to the cell an agent that modulates apoptosis-specific DHS function.
The method of claim 44, wherein the agent is an antisense oligonucleotide.
The method of claim 45, wherein the antisense oligonucleotide comprises a nucleotide sequence encoding a portion of an apoptosis-specific eIF-5A polypeptide.
An antisense oligonucleotide comprising a nucleotide sequence encoding a portion of an apoptosis-specific eIF-5A polypeptide.
An antisense oligonucleotide comprising a nucleotide sequence encoding a portion of an apoptosis-specific DHS polypeptide.
An expression vector comprising a promoter operably linked to a nucleotide sequence encoding an apoptosis-specific eIF-5A polypeptide.
An expression vector comprising a promoter operably linked to a nucleotide sequence encoding an apoptosis-specific DHS polypeptide.
A method of identifying modulation of apoptosis comprising:
Dependencies are inferred from the claim text.