Expression system
OwnerReuven Agami, Thijn Brummelkampinventors
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Abstract
The present invention provides a polynucleotide comprising a RNA polymerase III promoter, a region encoding a siRNA, and a transcriptional termination element comprising five consecutive thymine residues. The invention also provides for vectors, cells and non-human transgenic animal comprising the polynucleotides of the invention as well as their use in medicaments for various conditions.
Claims24
A polynucleotide comprising:
- a RNA polymerase III promoter;
- a region encoding a siRNA;
- and
- a transcriptional termination element comprising five consecutive thymine residues in the sense strand.
A polynucleotide according to any one of the preceding claims, wherein the siRNA is capable of discriminating between different alleles of the same gene.
A vector comprising a polynucleotide according to any one of the preceding claims.
Dependencies are inferred from the claim text.