Multiple CpG oligodeoxynucleotides and their use to induce an immune response
AssigneeThe Government of the U.S.A., The Secretary of the Department of Health and Human Services
InventorsDennis Klinman, Ken Ishii, Daniela Verthelyi
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Abstract
Compositions including multiple oligodeoxynucleotides with a CpG motif are disclosed herein. The compositions can include either D or K type oligodeoxynucleotides. These compositions are of use in inducing an immune response in a large percentage of the individuals in a population.
Claims48
An oligodeoxynucleotide composition comprising at least two oligodeoxynucleotides of at least about 10 nucleotides in length, wherein the oligodeoxynucleotides comprise an unmethylated CpG motif, and wherein the oligodeoxynucleotides comprises a sequence represented by the formula 5′ N1N2N3T-CpG-WN4N5N6 3′, wherein W is A or T, and N1, N2, N3, N4, N5, and N6 are any nucleotide;
- and wherein the at least two different oligodeoxynucleotides differ in their nucleic acid sequence.
An oligodeoxynucleotide composition comprising at least two oligodeoxynucleotide of at least about 10 nucleotides in length, wherein the oligodeoxynucleotides comprise an unmethylated CpG motif, and wherein at the oligodeoxynucleotides comprise a sequence represented by the formula 5′ RY-CpG-RY 3′, wherein R is A or G and Y is C or T;
- and the at least two oligodeoxynucleotides differ in their nucleic acid sequence.
The method of claim 46, wherein upon administration of the at least two oligodeoxynucleotides to a population of subjects, at least 80% of the subjects produces an immune response, and wherein the immune response comprises substantial stimulation of a parameter of the immune response as compared to administration of only one of the oligodeoxynucleotides to the population of subjects.
The method of claim 47, wherein upon administration of the at least two oligodeoxynucleotides to a population of subjects, at least two parameters of the immune response are stimulated in the subjects, wherein administration of only one of the oligodeoxynucleotides to the population of subjects stimulates only a single parameter of the immune response in some of the subjects in the population.
Dependencies are inferred from the claim text.