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AssigneeMonsanto Technology LLC
InventorsWilliam P. Donovan, Judith C. Donovan, Annette C. Slaney
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Disclosed are Bacillus thuringiensis strains comprising novel crystal proteins which exhibit insecticidal activity against coleopteran insects including red flour beetle larvae (Tribolium castaneum) and Japanese beetle larvae (Popillia japonica). Also disclosed are novel B. thuringiensis crystal toxin genes, designated cryET33 and cryET34, which encode the colepteran-toxic crystal proteins, CryET33 (29-kDa) crystal protein, and the cryET34 gene encodes the 14-kDa CryET34 crystal protein. The CryET33 and CryET34 crystal proteins are toxic to red flour beetle larvae and Japanese beetle larvae. Also disclosed are methods of making and using transgenic cells comprising the novel nucleic acid sequences of the invention.
An isolated and purified Bacillus thuringiensis CryET33 or CryET34 crystal protein.
An isolated and purified CryET33 or CryET34 crystal protein having insecticidal activity against the Japanese beetle (Popillia japonica) and the red flour beetle (Tribolium castaneum).
A purified nucleic acid segment encoding a CryET33 or a CryET34 crystal protein.
An isolated and purified DNA segment comprising a Bacillus thuringiensis cryET33 gene.
An isolated and purified DNA segment comprising a Bacillus thuringiensis cryET34 gene.
A method of using a DNA segment that encodes a CryET33 or CryET34 crystal protein or peptide, comprising the steps of:
An isolated nucleic acid segment characterized as:
A method for detecting a nucleic acid sequence encoding a CryET33 or CryET34 crystal protein, comprising the steps of:
A nucleic acid detection kit comprising, in suitable container means, a CryET33 or a CryET34 crystal protein-encoding nucleic acid segment and a detection reagent.
A peptide composition, comprising a CryET33 or CryET34 crystal protein that includes a five amino acid contiguous sequence from SEQ ID NO:
A purified antibody that binds to a CryET33 or CryET34 crystal protein or peptide.
A method for detecting a CryET33 or CryET34 crystal protein or peptide in a biological sample, comprising the steps of:
An immunodetection kit comprising, in suitable container means, an antibody that binds to a CryET33 or CryET34 crystal protein or peptide, and an immunodetection reagent.
A transgenic plant having incorporated into its genome a transgene that encodes a CryET33 or a CryET34 crystal protein or peptide.
A Bacillus thuringiensis cell that produces a CryET33 or a CryET34 crystal protein.
A Bacillus thuringiensis cell having the NRRL accession number B-21365.
A Bacillus thuringiensis cell having the NRRL accession number B-21366.
A Bacillus thuringiensis cell having the NRRL accession number B-21367.
A composition comprising from about 1% to about 50% by weight of a CryET33 or a CryET34 crystal protein.
A composition comprising a CryET33 or a CryET34 crystal protein prepared by a process comprising the steps of:
A method of preparing a CryET33 or a CryET34 crystal protein comprising:
Dependencies are inferred from the claim text.