US 2003/0144191 A1 · Polynucleotide encoding a novel TRP channel family member, TRP-PLIK2, and splice variants thereof

Abstract

The present invention provides novel polynucleotides encoding TRP-PLIK2 polypeptides, fragments and homologues thereof. The present invention also provides polynucleotides encoding variants and splice variants of TRP-PLIK2 polypeptides, TRP-PLIK2b, TRP-PLIK2c, and TRP-PLIK2d, respectively. Also provided are vectors, host cells, antibodies, and recombinant and synthetic methods for producing said polypeptides. The invention further relates to diagnostic and therapeutic methods for applying these novel TRP-PLIK2, TRP-PLIK2b, TRP-PLIK2c, and TRP-PLIK2d polypeptides to the diagnosis, treatment, and/or prevention of various diseases and/or disorders related to these polypeptides. The invention further relates to screening methods for identifying agonists and antagonists of the polynucleotides and polypeptides of the present invention.

An isolated nucleic acid molecule comprising a polynucleotide having a nucleotide sequence at least 95.0% identical to a sequence selected from the group consisting of:

(a) a polynucleotide fragment of SEQ ID NO:1 or a polynucleotide fragment of the cDNA sequence included in ATCC Deposit No:
PTA-4175, which is hybridizable to SEQ ID NO 1;
(b) a polynucleotide encoding a polypeptide fragment of SEQ ID NO:2 or a polypeptide fragment encoded by the cDNA sequence included in ATCC Deposit No:
PTA-4175, which is hybridizable to SEQ ID NO:1;
(c) a polynucleotide encoding a polypeptide domain of SEQ ID NO:2 or a polypeptide domain encoded by the cDNA sequence included in ATCC Deposit No:
PTA-4175, which is hybridizable to SEQ ID NO:1;
(d) a polynucleotide encoding a polypeptide epitope of SEQ ID NO:2 or a polypeptide epitope encoded by the cDNA sequence included in ATCC Deposit No:
PTA-4175, which is hybridizable to SEQ ID NO:1;
(e) a polynucleotide encoding a polypeptide of SEQ ID NO:2 or the cDNA sequence included in ATCC Deposit No:
PTA-4175, which is hybridizable to SEQ ID NO:1, having transient potential protein activity;
(f) an isolated polynucleotide comprising nucleotides 4 to 6051 of SEQ ID NO:1, wherein said nucleotides encode a polypeptide corresponding to amino acids 2 to 2017 of SEQ ID NO:2 minus the start codon;
(g) an isolated polynucleotide comprising nucleotides 1 to 6051 of SEQ ID NO:1, wherein said nucleotides encode a polypeptide corresponding to amino acids 1 to 2017 of SEQ ID NO:2 including the start codon;
(h) an isolated polynucleotide comprising nucleotides 904 to 1064 of SEQ ID NO:1, wherein said nucleotides encode an ion transport domain of the TRP-PLIK2 polypeptide of SEQ ID NO:2;
(j) a polynucleotide which represents the complimentary sequence (antisense) of SEQ ID NO:1;
(k) a polynucleotide fragment of SEQ ID NO:3 which is hybridizable to SEQ ID NO:3;
(l) a polynucleotide encoding a polypeptide fragment of SEQ ID NO:4 which is hybridizable to SEQ ID NO:3;
(m) a polynucleotide encoding a polypeptide domain of SEQ ID NO:4 which is hybridizable to SEQ ID NO:3;
(n) a polynucleotide encoding a polypeptide epitope of SEQ ID NO:4 which is hybridizable to SEQ ID NO:3;
(o) a polynucleotide encoding a polypeptide of SEQ ID NO:4 which is hybridizable to SEQ ID NO:3, having transient potential protein activity;
(p) an isolated polynucleotide comprising nucleotides 4 to 5910 of SEQ ID NO:3, wherein said nucleotides encode a polypeptide corresponding to amino acids 2 to 1970 of SEQ ID NO:4 minus the start codon;
(q) an isolated polynucleotide comprising nucleotides 1 to 5910 of SEQ ID NO:3, wherein said nucleotides encode a polypeptide corresponding to amino acids 1 to 1970 of SEQ ID NO:4 including the start codon;
(r) an isolated polynucleotide comprising nucleotides 857 to 1012 of SEQ ID NO:3, wherein said nucleotides encode an ion transport domain of the TRP-PLIK2b polypeptide of SEQ ID NO:4;
(s) a polynucleotide which represents the complimentary sequence (antisense) of SEQ ID NO:3;
(t) a polynucleotide fragment of SEQ ID NO:5 which is hybridizable to SEQ ID NO:5;
(u) a polynucleotide encoding a polypeptide fragment of SEQ ID NO:6 which is hybridizable to SEQ ID NO:5;
(v) a polynucleotide encoding a polypeptide domain of SEQ ID NO:6 which is hybridizable to SEQ ID NO:5;
(w) a polynucleotide encoding a polypeptide epitope of SEQ ID NO:6 which is hybridizable to SEQ ID NO:5;
(x) a polynucleotide encoding a polypeptide of SEQ ID NO:6 which is hybridizable to SEQ ID NO:5, having transient potential protein activity;
(y) an isolated polynucleotide comprising nucleotides 4 to 5817 of SEQ ID NO:5, wherein said nucleotides encode a polypeptide corresponding to amino acids 2 to 1939 of SEQ ID NO:6 minus the start codon;
(z) an isolated polynucleotide comprising nucleotides 1 to 5817 of SEQ ID NO:5, wherein said nucleotides encode a polypeptide corresponding to amino acids 1 to 1939 of SEQ ID NO:6 including the start codon;
(aa) an isolated polynucleotide comprising nucleotides 826 to 986 of SEQ ID NO:5, wherein said nucleotides encode an ion transport domain of the TRP-PLIK2c polypeptide of SEQ ID NO:6;
(bb) a polynucleotide which represents the complimentary sequence (antisense) of SEQ ID NO:5;
(cc) a polynucleotide fragment of SEQ ID NO:7 which is hybridizable to SEQ ID NO:7;
(dd) a polynucleotide encoding a polypeptide fragment of SEQ ID NO:8 which is hybridizable to SEQ ID NO:7;
(ee) a polynucleotide encoding a polypeptide domain of SEQ ID NO:8 which is hybridizable to SEQ ID NO:7;
(ff) a polynucleotide encoding a polypeptide epitope of SEQ ID NO:8 which is hybridizable to SEQ ID NO:7;
(gg) a polynucleotide encoding a polypeptide of SEQ ID NO:8 which is hybridizable to SEQ ID NO:7, having transient potential protein activity;
(hh) an isolated polynucleotide comprising nucleotides 4 to 5922 of SEQ ID NO:7, wherein said nucleotides encode a polypeptide corresponding to amino acids 2 to 1974 of SEQ ID NO:8 minus the start codon;
(ii) an isolated polynucleotide comprising nucleotides 1 to 5922 of SEQ ID NO:7, wherein said nucleotides encode a polypeptide corresponding to amino acids 1 to 1974 of SEQ ID NO:8 including the start codon;
(jj) an isolated polynucleotide comprising nucleotides 904 to 959 of SEQ ID NO:7, wherein said nucleotides encode an ion transport domain of the TRP-PLIK2d polypeptide of SEQ ID NO:8;
(kk) a polynucleotide which represents the complimentary sequence (antisense) of SEQ ID NO:7;
(ll) a polynucleotide fragment of SEQ ID NO:97 which is hybridizable to SEQ ID NO:97;
(mm) a polynucleotide encoding a polypeptide fragment of SEQ ID NO:98 which is hybridizable to SEQ ID NO:97;
(nn) a polynucleotide encoding a polypeptide domain of SEQ ID NO:98 which is hybridizable to SEQ ID NO:97;
(oo) a polynucleotide encoding a polypeptide epitope of SEQ ID NO:98 which is hybridizable to SEQ ID NO:97;
(pp) a polynucleotide encoding a polypeptide of SEQ ID NO:98 which is hybridizable to SEQ ID NO:97, having transient potential protein activity;
(qq) an isolated polynucleotide comprising nucleotides 29 to 1513 of SEQ ID NO:97, wherein said nucleotides encode a polypeptide corresponding to amino acids 2 to 496 of SEQ ID NO:98 minus the start codon;
(rr) an isolated polynucleotide comprising nucleotides 1 to 1513 of SEQ ID NO:97, wherein said nucleotides encode a polypeptide corresponding to amino acids 1 to 496 of SEQ ID NO:98 including the start codon;
(ss) a polynucleotide which represents the complimentary sequence (antisense) of SEQ ID NO:97;
and
(tt) a polynucleotide capable of hybridizing under stringent conditions to any one of the polynucleotides specified in (a)-(ss), wherein said polynucleotide does not hybridize under stringent conditions to a nucleic acid molecule having a nucleotide sequence of only A residues or of only T residues.

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Polynucleotide encoding a novel TRP channel family member, TRP-PLIK2, and splice variants thereof

Life of this patent

Polynucleotide encoding a novel TRP channel family member, TRP-PLIK2, and splice variants thereof

Life of this patent

Abstract

Claims19