Methods for identifying compounds useful for the regulation of body weight and associated conditions
OwnerMiles B. Brennan, Ute Hochgeschwenderinventors
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Abstract
Described are methods for identifying compounds useful for regulation of body weight and associated conditions. In particular, methods are disclosed for identification of compounds that preferentially bind to and/or activate peripheral melanocortin receptors and which minimize binding and/or activation of central melanocortin receptors.
Claims21
A method for identifying compounds that regulate body weight by preferentially regulating peripheral pathways of energy homeostasis, comprising:
- a. contacting a putative regulatory compound with a cell which expresses a melanocortin receptor selected from the group consisting of melanocortin 2-receptor (MC2-R) and melanocortin 5-receptor (MC5-R);
- b. detecting whether the putative regulatory compound increases said melanocortin receptor activity;
- c. contacting said putative regulatory compound with a cell which expresses a melanocortin 4-receptor (MC4-R);
- and,
- d. detecting whether the putative regulatory compound increases MC4-R activity;
- wherein putative regulatory compounds that induce greater MC2-R activity or MC5-R activity as compared to MC4-R activity are identified as compounds that regulate body weight by preferentially regulating peripheral pathways of energy homeostasis.
A method for identifying compounds that increase body weight by regulating peripheral pathways of energy homeostasis, comprising:
- a. contacting a cell which expresses a melanocortin receptor selected from the group consisting of melanocortin 2-receptor (MC2-R) and melanocortin 5-receptor (MC5-R) with a POMC compound which binds to and activates said melanocortin receptor in the presence and absence of a putative regulatory compound;
- b. detecting whether said putative regulatory compound inhibits said melanocortin receptor activity;
- wherein putative regulatory compounds that inhibit said melanocortin receptor activity are identified as compounds that increase body weight by regulating peripheral pathways of energy homeostasis.
A method for identifying compounds that regulate body weight by regulating peripheral pathways of energy homeostasis, comprising:
- a. contacting a putative regulatory compound with a cell which expresses a melanocortin receptor selected from the group consisting of melanocortin 2-receptor (MC2-R) and melanocortin 5-receptor (MC5-R);
- b. detecting whether the putative regulatory compound binds to said melanocortin receptor;
- c. administering compounds which bind to said melanocortin receptor to a non-human test animal and detecting whether the putative regulatory compound regulates the body weight of said test animal;
- wherein putative regulatory compounds that interact with the melanocortin receptor and that regulate the body weight of the test animal are identified as compounds which regulate body weight by regulating peripheral pathways of energy homeostasis.
A method for identifying compounds that increase body weight by regulating peripheral pathways of energy homeostasis, comprising:
- a. contacting a cell which expresses a melanocortin receptor selected from the group consisting of melanocortin 2-receptor (MC2-R) and melanocortin 5-receptor (MC5-R) with a POMC compound which binds to and activates said melanocortin receptor in the presence and absence of a putative regulatory compound;
- b. detecting whether said POMC compound binds to said melanocortin receptor;
- c. administering compounds which bind to said melanocortin receptor to a non-human test animal and detecting whether the putative regulatory compound regulates the body weight of said test animal;
- wherein putative regulatory compounds that bind to the melanocortin receptor and that regulate the body weight of the test animal are identified as compounds which increase body weight by regulating peripheral pathways of energy homeostasis.
A method for identifying compounds that regulate body weight by regulating peripheral pathways of energy homeostasis, comprising:
- a. contacting a putative regulatory compound with a cell or cell lysate containing a reporter gene operatively associated with a regulatory element of a melanocortin receptor selected from the group consisting of melanocortin 2-receptor (MC2-R) and melanocortin 5-receptor (MC5-R);
- b. detecting expression of the reporter gene product;
- c. contacting a putative regulatory compound with a cell or cell lysate containing a reporter gene operatively associated with a regulatory element of a melanocortin 4-receptor (MC4-R);
- and,
- d. detecting expression of the reporter gene product;
- wherein putative regulatory compounds that increase expression of the reporter gene product of (b) as compared to the reporter gene product of (d) are identified as compounds that regulate body weight by preferentially regulating peripheral pathways of energy homeostasis.
A method for identifying compounds that regulate body weight by regulating peripheral pathways of energy homeostasis, comprising:
- a. contacting a putative regulatory compound with a cell or cell lysate containing transcripts of a melanocortin receptor selected from the group consisting of melanocortin 2-receptor (MC2-R) and melanocortin 5-receptor (MC5-R);
- and,
- b. detecting translational inhibition of the melanocortin receptor transcript;
- wherein putative regulatory compounds that inhibit said melanocortin receptor transcript are identified as compounds that increase body weight by regulating peripheral pathways of energy homeostasis.
A method for identifying compounds that regulate peripheral pathways of energy homeostasis, comprising:
- (a) contacting a putative regulatory compound with an isolated adipocyte;
- and,
- (b) detecting putative regulatory compounds that bind to a melanocortin receptors on said adipocyte, wherein putative regulatory compounds that bind to melanocortin receptors on said adipocytes are identified as compounds that regulate body weight by regulating peripheral pathways of energy homeostasis.
Dependencies are inferred from the claim text.