Monoclonal antibodies directed against the microtubule-associated protein tau, hybridomas secreting these antibodies, antigen recognition by these monoclonal antibodies and their applications
AssigneeInnogenetics N.V.
InventorsMarc Vandermeeren, Eugeen Vanmechelen, Marc Mercken, Andre Van De Voorde
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Abstract
The invention relates to a monoclonal antibody which forms an immunological complex with an epitope of an antigen belonging to normal human tau protein as well as abnormally phosphorylated human tau protein, with said tau protein being liable to be obtained from a brain homogenate, itself isolated from human cerebral cortex. The monoclonal antibodies of the invention can be used to detect tau and abnormally phosphorylated tau in brain extracts and in unconcentrated cerebrospinal fluid.
Claims15
Monoclonal antibody which forms an immunological complex with an epitope of an antigen belonging to human normal as well as abnormally phosphorylated tau protein, with said tau protein being liable to be obtained from a brain homogenate, itself isolated from the human cerebral cortex, characterized by the fact that:
- it does not form an immunological complex with other phosphorylated proteins such as MAP-1, MAP-2 and neurofilaments which share part of their sequence with tau protein, as determined by means of an ELISA,
- and it is liable to detect human normal as well as abnormally phosphorylated tau protein in cerebrospinal fluid (CSF), with said tau protein being at a concentration as low as 1.0 pg/ml.
- and it is liable to detect said tau proteins with 100% recovery upon the addition of a fixed amount of tau proteins in tau-protein-negative CSF.
Dependencies are inferred from the claim text.