Transfection of eukaryontic cells with linear polynucleotides by electroporation
AssigneesUniversity of Antwerp, Gerold Schuler, Isolde Strobel
InventorsGerold Schuler, Zwi N. Berneman, Viggo F. I. Van Tendeloo, Peter Ponsaerts, Isolde Strobel
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Abstract
The present invention provides an improved method for gene delivery in eukaryotic cells by electroporation, preferably in human hematopoietic cells, particular dendritic cells. The method of the invention is superior to lipofection and passive pulsing of mRNA and to electroporation of plasmid cDNA for gene delivery, including tumor antigen loading of dendritic cells.
Claims17
A method for transfection of eukaryotic cells with one or more or a mixture of linear polynucleotides, which method comprises electroporation of a suspension containing the eukaryotic cells and the linear polynucleotides to be transfected at a capacitance of below 300 μF.
Dependencies are inferred from the claim text.