Processes for transposase mediated integration into mammalian cells
OwnerChristine Wooddell, Hans Herweijer +1inventors
InventorsChristine Wooddell, Hans Herweijer, Jon A. Wolff
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Abstract
We disclose compositions and processes for transferring a nucleic acid into a mammalian cell utilizing a transposase to achieve nonviral integration of exogenous nucleic acid into the chromosomal DNA of the cell.
Claims12
A process for integrating nucleic acid into the genome of mammalian cells comprising, forming an integrator complex between the nucleic acid containing a transposon and a transposase specific for the transposon;
- and, delivering the integrator complex to a mammalian cell.
A composition for integrating nucleic acids into a mammalian genome, comprising, a Tn5 integrator complex consisting of Tn5 transposase and a mammalian nucleic acid sequence.
A process for integrating a nucleic acid into amammalianchromosome, comprising:
- forming an integrator complex in solution;
- associating the complex with a transfection reagent;
- delivering the complex to a mammalian cell wherein the nucleic acid is integrated into the mammalian chromosome.
Dependencies are inferred from the claim text.