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AssigneeMonash University
InventorsJohn Roderick Morrison, Eric Shannon Hayes, Martin Frederick Pera, Orly Lacham-Kaplan, Alan Osborne Trounson
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The present invention generally relates to neural stem cells, preferably foetal neural stem cells and their progeny thereof. The present invention provides methods of isolating, culturing and propagating neural stem cells preferably foetal neural stem cells and the development of neural stem cell lines and lineages. The present invention also relates to the use of neural stem cells and somatic cells (eg rat fetal fibroblasts) and cells expressing the telomerase catalytic component (TERT) for gene targeting and gene knockout experiments and for producing genetically modified animals. In a first aspect of the present invention there is provided a cellular composition comprising one or more cells having a property characteristic of a neural stem cell and wherein said neural stem cell is capable of long term culture. Preferably the cells have a property characteristic of a foetal neural stem cell. In another aspect of the present invention there is provided a method of producing an animal, said method comprising introducing a continuously growing donor cell nucleus from a continuously growing donor cell into an oocyte or embryo and allowing the resulting embryo to mature and to preferably develop to a foetus or an adult animal.
A cellular composition comprising one or more cells having a property characteristic of a neural stem cell and wherein said neural stem cell is capable of long term culture.
A method of preparing a cellular composition comprising one or more cells having a property characteristic of a neural stem cell wherein said neural stem cell is capable of long term culture, said method comprising:
A method according to claim 8 wherein the source of the neural stem cell is a head or spinal cord of the foetus.
A method of producing an animal, said method comprising introducing a continuously growing donor cell nucleus from a continuously growing donor cell into an oocyte or embryo and allowing the resulting embryo to mature and to preferably develop to a foetus or animal.
A cell culture medium suitable for culturing neural stem cells in a long term culture comprising at least one lipid and at least one mitogenic factor.
A method of culturing neural stem cells said method comprising culturing the cells in the presence of at least one lipid and at least one mitogenic factor.
Dependencies are inferred from the claim text.