Loading patent…
OwnerAriffeen Bongso, Mark Richards +2inventors
InventorsAriffeen Bongso, Mark Richards, Fong Chui-Yee, Chan Woon-Khiong
Estimating the term…
The present invention relates to the field of stem cell culture, in particular undifferentiated stem cell culture and to methods for derivation and propagation of such cells. More particularly, the invention relates to derivation and propagation of undifferentiated HES cells on human feeder layers and/or in the absence of a feeder layer. The human feeder layers may be selected from the group including human fetal muscle (HFM), human fetal skin (HFS), human adult fallopian tubal (HAFT) fibroblasts and human adult skin cells. They may be cultured in the presence of a suitable medium selected from the group including Human Embryonic Stem Cell (HES), Knockout (KO), or Human Feeder (HF) medium supplemented with or without human serum.
A human feeder cell layer which supports the derivation of ES cells in a substantially undifferentiated state said feeder cell layer comprising cells selected from the group including human adult, fetal or embryonic cells or a combination thereof.
A human feeder cell layer which supports the culture of ES cells in a substantially undifferentiated state said feeder cell layer comprising cells selected from the group including human adult, fetal or embryonic cells or a combination thereof.
A human feeder cell layer according to claim 3 or 4 wherein the human fibroblast cell is a human adult fallopian tubal (HAFT) fibroblast cell.
A method of deriving an embryonic stem (ES) cell line in a substantially undifferentiated state from an ES cell population said method comprising:
A cell culture system for deriving and culturing ES cells in a substantially undifferentiated state, said culture system including:
Dependencies are inferred from the claim text.