Use of heterologous transcription factors in gene therapy
AssigneeARIAD Gene Therapeutics, Inc.
InventorsMichael Z. Gilman, Sridaran Natesan
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Abstract
This invention provides novel materials and methods involving the heterologous expression of transcription factors which are useful for effecting transcription of target genes in genetically engineered cells or organisms containing them. Target gene constructs and other materials useful for practicing the invention are also disclosed.
Claims37
A method for expressing a target gene in a cell within a host organism which comprises introducing into the organism cells containing:
- (a) a transcription factor construct containing a first heterologous DNA sequence encoding and capable of expressing a transcription factor capable of activating transcription of a gene linked to a transcription control sequence responsive to the transcription factor, and
- (b) a target gene construct containing a second heterologous DNA sequence comprising a target gene operably linked to a transcription control sequence comprising a DNA promoter sequence and one or more copies of a DNA recognition sequence permitting gene transcription responsive to the presence of the transcription factor.
A method for expressing a target gene in a cell within a host organism which comprises introducing into the organism, under conditions permitting DNA uptake by one or more cells within the organism:
- (a) a transcription factor construct containing a first heterologous DNA sequence encoding and capable of expressing a transcription factor capable of activating transcription of a gene linked to a transcription control sequence responsive to the transcription factor, and
- (b) a target gene construct containing a second heterologous DNA sequence comprising a target gene operably linked to a transcription control sequence comprising a DNA promoter sequence and one or more copies of a DNA recognition sequence permitting gene transcription responsive to the presence of the transcription factor.
A recombinant DNA sequence encoding a chimeric transcription factor containing one or more copies of peptide sequence comprising all or part of the peptide sequence spanning positions 361 through 550 of human NF-kB p65, or a peptide sequence derived therefrom, and peptide sequence heterologous thereto.
Dependencies are inferred from the claim text.