Improved Methods for producing oocysts
AssigneeEmbrex, Inc.
InventorsJames E. Hutchins, Julius K. Tyczkowski
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Abstract
The present invention provides improved methods and compositions for producing oocysts. The oocysts produced according to the invention find use in the manufacture of vaccines. In preferred embodiments, the present invention provides methods and compositions for the production of Eimeria oocysts. Vaccines containing Eimeria oocysts, sporocysts and/or sporozoites produced according to the present invention may be used to immunize birds against coccidiosis either in ovo or post hatch.
Claims69
A composition for the production of oocysts comprising a peroxygen compound and an organic acid, said composition having an acidic pH.
A composition for the production of oocysts comprising a peroxygen compound, said composition having an acidic pH.
A composition for the production of oocysts comprising an organic acid, said composition having an acidic pH.
A flotation medium for purifying protozoan oocysts, comprising a high-density, non-ionic solution and a polycation.
A flotation medium for purifying protozoan oocysts, comprising a high-density, non-ionic solution and oil.
A method of producing protozoan oocyts from an avian subject, comprising the steps of:
- (a) infecting an avian subject with a protozoan for a time sufficient for oocysts from the protozoan to be shed in the feces of the infected avian subject,
- (b) collecting the feces comprising the oocysts from the infected avian subject, and
- (c) feeding the infected avian subject a diet having a large mean particle size for at least about 1 day prior to and during said collecting step.
An aqueous composition for reducing aggregation among protozoan oocysts comprising a compound selected from the group consisting of a protein, a peptide, an amino acid, a protein hydrolysate, an anti-foaming agent, and any combination of the foregoing.
A method of producing protozoan oocysts, comprising a step of contacting a preparation of protozoan oocysts with an aqueous composition that reduces aggregation among the oocysts.
Dependencies are inferred from the claim text.