Histone deacetylase and methods of use thereof
AssigneeThe J. David Gladstone Institutes
InventorsEric Verdin, Wolfgang Fischle, Franck O. Dequiedt
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Abstract
The present invention provides nucleic acid molecules that encode histone deacetylase, as well as recombinant vectors and host cells that include the subject nucleic acid molecules. Also provided are histone deacetylase polypeptide compositions. The histone deacteylase nucleic acid molecules are useful in a variety of diagnostic and therapeutic applications, which are also provided.
Claims14
A histone deacetylase present in other than its natural environment, wherein said histone deacetylase has at least about 70% amino acid sequence identity to the amino acid sequence set forth in SEQ ID NO:02.
A nucleic acid molecule present in other than its natural environment, wherein said nucleic acid molecule has a nucleotide sequence that has at least about 70% nucleotide sequence identity to the coding region of the nucleotide sequence of SEQ ID NO:01.
An isolated nucleic acid molecule that hybridizes under stringent hybridization conditions to a nucleic acid comprising the coding region of SEQ ID NO:01 or its complementary sequence.
An expression cassette comprising a transcriptional initiation region functional in an expression host, a nucleic acid molecule encoding a polypeptide having at least 70% amino acid sequence identity to SEQ ID NO:02, said nucleic acid molecule under the transcriptional regulation of said transcriptional initiation region, and a transcriptional termination region functional in said expression host.
An in vitro method of identifying an agent that reduces enzymatic activity of histone deacetylase-7, comprising:
- a) contacting a histone deacetylase-7 with a test agent;
- and
- b) determining the effect, if any, of the test agent on histone deacetylase-7 enzyme activity.
A method for reducing tumor cell growth, comprising:
- contacting the tumor cell with an agent that reduces histone deacetylase-7 enzyme activity, wherein said tumor is characterized by an elevated level of histone deacetylase-7 compared to a normal cell of the same cell type.
A method for inducing apoptosis in a tumor cell, comprising:
- contacting the tumor cell with an agent that reduces histone deacetylase-7 enzyme activity, wherein said tumor is characterized by an elevated level of histone deacetylase-7 compared to a normal cell of the same cell type.
A method of identifying a cell, in a mixed population of cells, that expresses histone deacetylase-7 mRNA, the method comprising:
- a) contacting the cells with a nucleic acid molecule that specifically hybridizes to histone deacetylase-7 mRNA;
- and
- b) detecting hybridization.
A method of identifying a cell, in a mixed population of cells, that expresses histone deacetylase-7 mRNA, the method comprising:
- a) contacting the cells with an antibody that specifically binds to histone deacetylase-7.
Dependencies are inferred from the claim text.