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AssigneeCommonwealth Scientific and Industrial Research Organisation
InventorsRobyn Joyce Russell, Richard David Newcomb, Peter Malcolm Campbell, Geoffrey Charles de Quetteville Robin, Charles Claudianos, Kerrie-Ann Smyth, Thomas Mark Boyce, John Graham Oakeshott, Jeremy Colin Brownlie
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The present invention provides an isolated DNA molecule encoding Malathion Carboxylesterase capable of hydrolysing at least one organophosphate selected from the group consisting of carboxylester organophosphates and dimethylol organophosphates. The DNA molecule comprises a nucleotide sequence having at least 60%, preferably at least 80% and more preferably at least 95% homology with Lc?E 7, in which the protein encoded by the DNA molecule differs from E 3 at least in the substitution of Trip at position 251 with an amino acid selected from the group consisting of Leu, Ser, Ala, Ile, Val, Thr, Cys, Met and Gly. The preferred substituents are Leu and Ser.
An isolated DNA molecule encoding an enzyme capable of hydrolysing at least one organophosphate selected from the group consisting of carboxylester organophosphates and dimethyl-oxon organophosphates, the DNA molecule comprising a nucleotide sequence having at least 60% homology with LcαE7, in which the protein encoded by the DNA molecule differs from E3 at least in the substitution of Trp at position 251 with an amino acid selected from the group consisting of Leu, Ser, Ala, Ile, Val, Thr, Cys, Met and Gly.
An isolated DNA molecule, the DNA molecule encoding a polypeptide having the amino acid sequence of RM-8Con shown in FIG. 1 or the amino acid sequence of MdαE7 shown in FIG. 3 in which Trp at position 251 is replaced with Ser.
Dependencies are inferred from the claim text.