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US 2003/0143705 A1Published application
IovE variant regulator molecules
AssigneeMicrobia, Inc.
InventorsShannon Roberts, Amir Sherman, Joshua Trueheart, G. Todd Milne
Life of this patent
Estimating the term…
Abstract
The invention provides variant regulator proteins of secondary metabolite production and nucleic acids encoding said variant regulator proteins. In particular, the invention provides variant regulator molecules of the lovE protein.
Claims76
A protein comprising an amino acid sequence that codes for a variant protein of the lovE protein having at least one mutation selected from the group consisting of:
- (a) a Group 6 amino acid residue mutated to a Group 2 amino acid residue at position 31;
- (b) a Group 3 amino acid residue mutated to a Group 5 amino acid residue at position 41;
- (c) a Group 4 amino acid residue mutated to a Group 2 amino acid residue at position 52;
- (d) a Group 4 amino acid residue mutated to a Group 3 amino acid residue at position 52;
- (e) a Group 4 amino acid residue mutated to a Group 5 amino acid residue at position 73;
- (f) a Group 1 amino acid residue mutated to a Group 4 amino acid residue at position 101;
- (g) a Group 1 amino acid residue mutated to a Group 3 amino acid residue at position 101;
- (h) a valine amino acid residue mutated to another Group 2 amino acid residue at position 111;
- (i) a Group 4 amino acid residue mutated to a Group 2 amino acid residue at position 133;
- (j) a Group 3 amino acid residue mutated to a Group 2 amino acid residue at position 141;
- (k) a Group 3 amino acid residue mutated to a Group 5 amino acid residue at position 141;
- (l) a Group 4 amino acid residue mutated to Group 6 amino acid residue at position 153;
- (m) a Group 4 amino acid residue mutated to a Group 5 amino acid residue at position 153;
- (n) a Group 4 amino acid residue mutated to a Group 1 amino acid residue at position 281;
- (o) a Group 3 amino acid residue mutated to a Group 2 amino acid residue at position 367;
- (p) a Group 3 amino acid residue mutated to a Group 6 amino acid residue at position 367;
- (q) a Group 1 amino acid residue mutated to Group 4 amino acid residue at position 389;
- and
- (r) a Group 1 amino acid residue mutated to a Group 2 amino acid residue at position 389.
16 more elements
A nucleic acid comprising a polynucleotide sequence encoding an amino acid sequence of a variant protein of the lovE protein having at least one mutation selected from the group consisting of:
- (a) a Group 6 amino acid residue mutated to a Group 2 amino acid residue at position 31;
- (b) a Group 3 amino acid residue mutated to a Group 5 amino acid residue at position 41;
- (c) a Group 4 amino acid residue mutated to a Group 2 amino acid residue at position 52;
- (d) a Group 4 amino acid residue mutated to a Group 3 amino acid residue at position 52;
- (e) a Group 4 amino acid residue mutated to a Group 5 amino acid residue at position 73;
- (f) a Group 1 amino acid residue mutated to a Group 4 amino acid residue at position 101;
- (g) a Group 1 amino acid residue mutated to a Group 3 amino acid residue at position 101;
- (h) a valine amino acid residue mutated to another Group 2 amino acid residue at position 111;
- (i) a Group 4 amino acid residue mutated to a Group 2 amino acid residue at position 133;
- (j) an Group 3 amino acid residue mutated to a Group 2 amino acid residue at position 141;
- (k) an Group 3 amino acid residue mutated to a Group 5 amino acid residue at position 141;
- (l) a Group 4 amino acid residue mutated to Group 6 amino acid residue at position 153;
- (m) a Group 4 amino acid residue mutated to a Group 5 amino acid residue at position 153;
- (n) a Group 4 amino acid residue mutated to a Group 1 amino acid residue at position 281;
- (o) a Group 3 amino acid residue mutated to a Group 2 amino acid residue at position 367;
- (p) a Group 3 amino acid residue mutated to a Group 6 amino acid residue at position 367;
- (q) a Group 1 amino acid residue mutated to Group 4 amino acid residue at position 389;
- and
- (r) a Group 1 amino acid residue mutated to a Group 2 amino acid residue at position 389.
16 more elements
Dependencies are inferred from the claim text.