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OwnerSang-Yup Lee, Young Leeinventors
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The present invention provides recombinant plasmids containing a gene coding for polyhydroxyalkanoate (PHA) biosynthesis enzyme and a gene coding for intracellular PHA depolymerase, and a process for preparing (R)-hydroxycarboxylic acid employing the same. In accordance with the present invention, optically pure (R)-hydroxycarboxylic acid can be prepared by culturing E. coli transformed with a recombinant plasmid which expresses intracellular PHA depolymerase of Ralstonia eutropha and PHA biosynthesis enzyme of Alcaligenes latus to give (R)-hydroxycarboxylic acid. The process for preparing (R)-hydroxycarboxylic acid of the invention can be successfully employed in large-scale continuous process with a high productivity by carrying out PHA synthesis and degradation in a simultaneous manner, while enjoying the benefits of simple procedures for harvesting cells and disposing the cell wastes.
A recombinant plasmid comprising a nucleotide sequence encoding a polyhydroxyalkanoate (PHA) biosynthesis enzyme and a nucleotide encoding a PHA depolymerase.
A bacterial cell comprising a recombinant plasmid, wherein the bacterial cell is Escherichia coli XLI-Blue, and wherein the recombinant plasmid is pJC4Red.
A bacterial cell comprising a recombinant plasmid, wherein the bacterial cell is Escherichia coli XLI-Blue, and wherein the recombinant plasmid is pSYLI05Red
A bacterial cell comprising a recombinant plasmid, wherein the bacterial cell is Escherichia coli XLI-Blue, and wherein the recombinant plasmid is pSYL105Red-trc.
Dependencies are inferred from the claim text.