Human chemokine beta-13
AssigneeHuman Genome Sciences, Inc.
InventorsHaodong Li, George Seibel, Stephen Ullrich, Francis W. Luscinskas
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Abstract
The present invention relates to a novel CKβ-13 protein which is a member of the chemokine family. In particular, isolated nucleic acid molecules are provided encoding the human CKβ-13 protein. CKβ-13 polypeptides are also provided as are vectors, host cells and recombinant methods for producing the same. The invention further relates to screening methods for identifying agonists and antagonists of CKβ-13 activity. Also provided are diagnostic methods for detecting immune system-related disorders and therapeutic methods for treating immune system-related disorders.
Claims24
An isolated nucleic acid molecule nucleic acid molecule comprising a polynucleotide having a nucleotide sequence at least 95% identical to a sequence selected from the group consisting of:
- (a) a nucleotide sequence encoding the CKβ-13 polypeptide having the complete amino acid sequence in SEQ ID NO:
- 2;
- (b) a nucleotide sequence encoding the CKβ-13 polypeptide encoded by the cDNA clone contained in ATCC Deposit No. 97113;
- (c) a nucleotide sequence encoding a mature form of the CKβ-13 polypeptide having the amino acid sequence at positions 25-93 in SEQ ID NO:
- 2;
- (d) a nucleotide sequence encoding a mature form of the CKβ-13 polypeptide having the amino acid sequence at positions 29-93 in SEQ ID NO:
- 2;
- (e) a nucleotide sequence encoding a mature form of the CKβ-13 polypeptide as encoded by the cDNA clone contained in the ATCC Deposit No. 97113;
- and
- (f) a nucleotide sequence complementary to any of the nucleotide sequences in (a), (b), (c), (d) or (e) above.
An isolated nucleic acid molecule comprising a polynucleotide having a nucleotide sequence at least 95% identical to a sequence selected from the group consisting of:
- (a) a nucleotide sequence encoding a polypeptide comprising the amino acid sequence of residues n-93 of SEQ ID NO:
- 2, where n is an integer in the range of 1-35;
- (b) a nucleotide sequence encoding a polypeptide comprising the amino acid sequence of residues 1-m of SEQ ID NO:
- 2, where m is an integer in the range of 77-93;
- (c) a nucleotide sequence encoding a polypeptide having the amino acid sequence consisting of residues n-m of SEQ ID NO:
- 2, where n and m are integers as defined respectively in (a) and (b) above;
- and
- (d) a nucleotide sequence encoding a polypeptide consisting of a portion of the complete CKβ-13 amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97113 wherein said portion excludes from 1 to about 35 amino acids from the amino terminus of said complete amino acid sequence;
- (e) a nucleotide sequence encoding a polypeptide consisting of a portion of the complete CKβ-13 amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97113 wherein said portion excludes from 1 to about 17 amino acids from the carboxy terminus of said complete amino acid sequence;
- and
- (f) a nucleotide sequence encoding a polypeptide consisting of a portion of the complete CKβ-13 amino acid sequence encoded by the cDNA clone contained in ATCC Deposit No. 97113 wherein said portion include a combination of any of the amino terminal and carboxy terminal deletions in (d) and (e), above.
An isolated CKβ-13 polypeptide comprising an amino acid sequence at least 95% identical to a sequence selected from the group consisting of:
- (a) the complete amino acid sequence in SEQ ID NO:
- 2 or as encoded by the cDNA clone contained in ATCC Deposit No. 97113;
- and
- (b) the amino acid sequence of a mature CKβ-13 polypeptide having the amino acid sequence at positions 25-93 or 29-93 in SEQ ID NO:
- 2, or as encoded by the cDNA clone contained in the ATCC Deposit No. 97113.
An isolated polypeptide comprising an epitope-bearing portion of the CKβ-13 protein, wherein said portion is selected from the group consisting of:
- a polypeptide comprising amino acid residues from about Thr-22 to about Gly-28 (SEQ ID NO:
- 2);
- a polypeptide comprising amino acid residues from about Asn-30 to about Leu-47 (SEQ ID NO:
- 2);
- a polypeptide comprising amino acid residues from about Thr-56 to about Val-65 (SEQ ID NO:
- 2);
- and a polypeptide comprising amino acid residues from about Phe-70 to about Trp-83 (SEQ ID NO:
- 2).
An isolated polynucleotide comprising a nucleic acid encoding a polypeptide fragment of SEQ ID NO:
- 2;
- wherein said fragment has the same biological function or activity as CKβ-13.
Dependencies are inferred from the claim text.