Long-acting follicle stimulating hormone analogues and uses thereof
AssigneeThe Trustees of Columbia University in the City of New York
InventorJoyce Lustbader
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Abstract
This invention provides FSH analogues having increased serum half-life relative to FSH. This invention also provides related compositions and methods for increasing fertility, egg production and spermatogenesis in a subject.
Claims74
A synthetic FSH comprising a β-FSH subunit, an α-FSH subunit and a half-life-increasing moiety, wherein the β-FSH subunit, α-FSH subunit and half-life-increasing moiety are covalently bound.
A synthetic FSH comprising β-FSH subunit covalently bound to a polypeptide segment comprising the amino acid sequence ser-gly-ser-asn-ala-thr-gly-ser-gly-ser-asn-ala-thr-ser-gly-ser.
A synthetic FSH comprising a β-FSH subunit, an α-FSH subunit and a polypeptide segment comprising the amino acid sequence ser-gly-ser-asn-ala-thr-gly-ser-gly-ser-asn-ala-thr-ser-gly-ser, wherein the β-FSH subunit, α-FSH subunit and polypeptide segment are covalently bound.
A method for producing a synthetic FSH, which method comprises:
- (a) co-expressing (i) a nucleic acid which encodes an α-FSH subunit, and (ii) a nucleic acid which encodes a polypeptide comprising a β-FSH subunit and a polypeptide segment comprising the amino acid sequence ser-gly-ser-asn-ala-thr-gly-ser-gly-ser-asn-ala-thr-ser-gly-ser, under conditions permitting such co-expression;
- and
- (b) recovering the synthetic FSH so produced.
A method of increasing the half-life of a molecule in a subject, which method comprises glycosylating the molecule in a manner effective to increase the molecule's half-life.
Dependencies are inferred from the claim text.