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US 2003/0143685 A1Published application
Efficient protein expression system
AssigneeId Biomedical Corporation of Washington
InventorMary ChaoHong Hu
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Abstract
Nucleic acid expression control sequence cassettes and vectors containing the same are provided for use in making abundant quantities of recombinant polypeptides of interest. The modified transcriptional control sequences, which include a T5 promoter sequence, are highly stable and can be used in a variety of vectors, such as plasmids.
Claims20
A nucleic acid expression control sequence cassette, comprising:
- a) a transcription initiation sequence capable of remaining hybridized under stringent conditions to a T5 promoter sequence, wherein said transcription initiation sequence has at least basal T5 promoter transcriptional activity;
- b) at least one regulatory sequence operably linked to said transcription sequence of (a) and capable of remaining hybridized under stringent conditions to a lac operator sequence, wherein said at least one regulatory sequence specifically binds a lacI repressor protein and thereby alters transcriptional activity;
- c) at least one mutated regulatory sequence of (b) wherein said at least one mutated regulatory sequence does not specifically bind a lacI repressor protein and thereby does not alter transcriptional activity;
- and
- d) a translation initiation sequence.
2 more elements
Dependencies are inferred from the claim text.