US 2003/0143679 A1 · Genes encoding insect odorant receptors and uses thereof

Abstract

This invention provides an isolated nucleic acid molecule encoding an insect odorant receptor. This invention provides a nucleic acid molecule of at least 12 nucleotides capable of specifically hybridizing with the nucleic acid molecule encoding an insect odorant receptor. This invention also provides a purified, insect odorant receptor. This invention provides an antibody capable of specifically binding to an insect odorant receptor. This invention provides a method for identifying cDNA inserts encoding an insect odorant receptors. This invention provides a method of identifying a compound capable of specifically bind to an insect odorant receptor. This invention also provides a method of identifying a compound capable of activating the activity of an insect odorant receptor.

Claims54

Dependent claims

An isolated nucleic acid encoding a polypeptide present in an insect odorant receptor which polypeptide comprises seven transmembrane domains and a C-terminal domain, wherein one of the seven transmembrane domains is located within the polypeptide at a position adjoining the C-terminal domain and wherein this seventh transmembrane domain and the adjoining C-terminal domain together comprise consecutive amino acids the sequence of which is as follows:

10 (SEQ ID NO:
107) -(F, Y, L, A, T, S or C)-(P, I, M, V, T, L, Q, S or H)-(F, Y, I, S, L, C, M or V)-(C, Y, T, S, L or A)-(Y, N, F, M, I, L, K, S, H or T)-(X)20-W-;
wherein each X in (X)20 represents an amino acid and the identity of each X is independent of the identity of any other X.

An isolated nucleic acid encoding a polypeptide present in an insect odorant receptor, wherein the polypeptide is selected from the group consisting of polypeptides comprising consecutive amino acids the sequence of which is one of the following:

(a) SEQ ID NO:
2,
(b) SEQ ID NO:
4,
(c) SEQ ID NO:
6,
(d) SEQ ID NO:
8,
(e) SEQ ID NO:
10,
(f) SEQ ID NO:
12,
(g) SEQ ID NO:
14,
(h) SEQ ID NO:
16,
(i) SEQ ID NO:
18,
(j) SEQ ID NO:
20,
(k) SEQ ID NO:
22,
(l) SEQ ID NO:
24,
(m) SEQ ID NO:
26,
(n) SEQ ID NO:
28,
(o) SEQ ID NO:
30,
(p) SEQ ID NO:
32,
(q) SEQ ID NO:
34,
(r) SEQ ID NO:
36,
(s) SEQ ID NO:
38,
(t) SEQ ID NO:
40,
(u) SEQ ID NO:
42,
(v) SEQ ID NO:
44,
(w) SEQ ID NO:
46,
(x) SEQ ID NO:
48,
(y) SEQ ID NO:
50,
(z) SEQ ID NO:
52,
(aa) SEQ ID NO:
54,
(bb) SEQ ID NO:
56,
(cc) SEQ ID NO:
58,
(dd) SEQ ID NO:
60,
(ee) SEQ ID NO:
62,
(ff) SEQ ID NO:
64,
(gg) SEQ ID NO:
66,
(hh) SEQ ID NO:
68,
(ii) SEQ ID NO:
70,
(jj) SEQ ID NO:
72,
(kk) SEQ ID NO:
74,
(ll) SEQ ID NO:
76,
(mm) SEQ ID NO:
78,
(nn) SEQ ID NO:
80,
(oo) SEQ ID NO:
82,
(pp) SEQ ID NO:
84,
(qq) SEQ ID NO:
86,
(rr) SEQ ID NO:
88,
(ss) SEQ ID NO:
90,
(tt) SEQ ID NO:
92,
(uu) SEQ ID NO:
94,
(vv) SEQ ID NO:
96,
(ww) SEQ ID NO:
98,
(xx) SEQ ID NO:
100,
(yy) SEQ ID NO:
102,
(zz) SEQ ID NO:
104,
(aaa) SEQ ID NO:
106, or
(bbb) a polypeptide which shares greater than 25% amino acid identity with any one of the polypeptides of (a)-(aaa), and comprises a transmembrane domain and an adjoining C-terminal domain which together comprise consecutive amino acids the sequence of which is as follows:
13 (SEQ ID NO:
107) -(F, Y, L, A, T, S or C)-(P, I, M, V, T, L, Q, S or H)-(F, Y, I, S, L, C, M or V)-(C, Y, T, S, L or A)-(Y, N, F, M, I, L, K, S, H or T)-(X)20-W-;
wherein each X in (X)20 represents an amino acid and the identity of each X is independent of the identity of any other X.

107 more elements

Dependencies are inferred from the claim text.

Genes encoding insect odorant receptors and uses thereof

Life of this patent

Genes encoding insect odorant receptors and uses thereof

Life of this patent

Abstract

Claims54