Activated rec-D-hydantoinases
AssigneeDegusa AG
InventorsOliver May, Andreas Bommarius, Karlheinz Drauz, Martin Siemann-Herzberg, Christoph Syldatk, Markus Werner, Josef Altenbuchner
Life of this patent
Estimating the term…
Abstract
Rec-hydantoinases which may be obtained in more active form by a the process described herein. The invention also relates, inter alia, to a rechydantoinase from the organism Arthrobacter crystallopoietes DSM20117, to nucleic acids which code for such a protein and to vectors containing said nucleic acids and to uses thereof.
Claims24
A process for producing an activated rec-hydantoinase, comprising fermenting a microorganism which produces a rec-hydantoinase in the presence of a concentration of at least one divalent metal ion sufficient to activate the rec-hydantoinase.
An activated rec-hydantoinase obtained by a process comprising fermenting a microorganism which produces a rec-hydantoinase in the presence of a concentration of at least one divalent metal ion sufficient to activate the rec-hydantoinase.
An isolated nucleic acid which codes for a D-hydantoinase from Arthrobacter crystallopoietes DSM 20117.
A process for the producing an improved rec-hydantoinase, comprising:
- (a) mutagenizing a nucleic acid which codes for a rec-hydantoinase,
- (b) cloning the mutangenized nucleic acids from (a) into a vector,
- (c) transferring the vector from (b) into an expression system,
- (d) expressing the nucleic acid in the expression system,
- (e) detecting protein which have improved activity and/or selectivity, and
- (f) isolating the protein detected in (e).
A process for the producing a nucleic acid which encodes an improved rec-hydantoinase, comprising:
- (a) mutagenizing a nucleic acid which codes for a rec-hydantoinase,
- (b) cloning the mutangenized nucleic acids from (a) into a vector,
- (c) transferring the vector from (b) into an expression system,
- (d) expressing the nucleic acid in the expression system,
- (e) detecting protein which have improved activity and/or selectivity, and
- (f) isolating the nucleic acid which encodes the protein detected in (e).
Dependencies are inferred from the claim text.