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US 2003/0143672 A1Published application
Heteroclitic analogs and related methods
AssigneeEpimmune Inc.
InventorsShabnam Tangri, Alessandro Sette, Glenn Ishioka, John D. Fikes
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Abstract
Heteroclitic analogs of Class I epitopes are prepared by providing conservative or semi-conservative amino acid substitutions at positions 3 and/or 5 and/or 7 of these epitopes. The analogs are useful in eliciting immune responses with respect to the corresponding wildtype epitopes.
Claims39
A method of producing a polypeptide comprising an analog of a Major Histocompatibility Complex (MHC) class I peptide epitope,
- wherein said analog has enhanced immunogenicity compared to said epitope, said method comprising:
- (a) identifying a MHC class I epitope comprising a formula (A),
- wherein formula (A) is Rn-R2-R3-R4-R5-R6-R7- . . . Rx,
- Rn is the N-terminal amino acid,
- Rx is the C-terminal amino acid,
- x=8-11 such that Rx can be from the eighth to the eleventh amino acid residue from Rn,
- R2 or R3 and Rx are primary anchor residues of a motif or supermotif, and
- (b) producing a polypeptide comprising an analog, said analog comprising a formula (B) identical to said formula (A) except one or more conservative or semiconservative amino acid substitutions at R3 and/or R5 and/or R7, provided said one or more substitutions is not of a primary anchor residues.
5 more elements
A method of producing a polypeptide comprising an analog of a Major Histocompatibility Complex (MHC) class I peptide epitope,
- wherein said analog has enhanced immunogenicity compared to said epitope, said method comprising:
- (a) identifying a MHC class I epitope comprising a formula (A),
- wherein formula (A) is Rn-R2-R3-R4-R5-R6-R7- . . . Rx,
- Rn is the N-terminal amino acid,
- Rx is the C-terminal amino acid,
- x=8-11 such that Rx can be from the eighth to the eleventh amino acid residue from Rn,
- R2 and Rx are primary anchor residues of a B7 supermotif, and
- (b) producing a polypeptide comprising an analog, said analog comprising a formula (B) identical to said formula (A) except that one or more than one of R3 and/or R5 and/or R7 comprise a non-conservative amino acid substitution.
5 more elements
Dependencies are inferred from the claim text.