Genetic locus for everninomicin biosynthesis
OwnerAlfredo Staffa, Emmanuel Zazopoulos +3inventors
InventorsAlfredo Staffa, Emmanuel Zazopoulos, Stephane Mercure, Piotr Peter Nowacki, Chris M. Farnet
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Abstract
The present invention relates to isolated genetic sequences encoding proteins which direct the biosynthesis of the antibiotic everninomicin in Micromonospora carbonacea. The isolated biosynthetic gene cluster serves as a substrate for bioengineering of antibiotic structures.
Claims20
An isolated nucleic acid molecule comprising a nucleic acid sequence selected from any of:
- (a) a nucleic acid encoding any of everninomicin open reading frames (ORFs) 1 to 49 (SEQ ID NOS:
- 2, 5 to 7, 9 to 21, 23 to 35, 37 to 46, 48, and 50 to 58);
- (b) a nucleic acid encoding a polypeptide encoded by any of everninomicin open reading frames (ORFS) 1 to 49 (SEQ ID NOS:
- 2, 5 to 7, 9 to 21, 23 to 35, 37 to 46, 48, and 50 to 58);
- and
- (c) a nucleic acid encoding a polypeptide which is at least 75% identical in amino acid sequence to a polypeptide encoded by any of everninomicin open reading frames (ORFs) 1 to 49 (SEQ ID NOS:
- 2, 5 to 7, 9 to 21, 23 to 35, 37 to 46, 48, and 50 to 58).
An isolated nucleic acid comprising a nucleic acid that hybridizes under stringent conditions to an open reading frame (ORF) of the everninomicin biosynthesis gene cluster and can substitute for the ORF to which it specifically hybridizes to direct the synthesis of an everninomicin.
An isolated gene cluster comprising open reading frames encoding polypeptides sufficient to direct the synthesis of an everninomicin or an everninomicin analogue.
An isolated polypeptide comprising a polypeptide sequence selected from any one of:
- a) a polypeptide of open reading frames 1 to 49 (SEQ ID NOS:
- 2, 5 to 7, 9 to 21, 23 to 35, 37 to 46, 48, and 50 to 58);
- and
- b) a polypeptide which is at least 75% identical in amino acid sequence to a polypeptide sequence of open reading frames (ORFs) 1 to 49 (SEQ ID NOS:
- 2, 5 to 7, 9 to 21, 23 to 35, 37 to 46, 48, and 50 to 58).
A method of chemically modifying a biological molecule, said method comprising contacting a biological molecule that is a substrate for a polypeptide encoded by an everninomicin biosynthesis gene cluster open reading frame with a polypeptide encoded by an everninomicin biosynthesis gene cluster open reading frame whereby said polypeptide chemically modifies said biological molecule.
Dependencies are inferred from the claim text.