Product and process to regulate actin polymerization in T lymphocytes
AssigneeNational Jewish Medical and Research Center
InventorsTerri H. Finkel, Moshe M. Rozdzial
Life of this patent
Estimating the term…
Abstract
The present invention relates to methods to regulate actin polymerization in T lymphocytes involved in tumorigenesis, inflammatory responses, immune responses, allergic responses and graft rejection responses, kits to perform such assays and methods to identify regulatory reagents that specifically control actin polymerization in T lymphocytes.
Claims45
A method to identify compounds capable of regulating actin polymerization in a T lymphocyte, comprising:
- (a) contacting a putative regulatory compound with a T lymphocyte having a T cell receptor chain selected from the group consisting of a zeta chain and an epsilon chain, to form a contacted lymphocyte;
- (b) combining said contacted lymphocyte with a molecule capable of inducing the phosphorylation of said zeta chain or said epsilon chain;
- and
- (c) assessing the ability of said putative regulatory compound to regulate actin polymerization in said lymphocyte.
A method to regulate actin polymerization in a T lymphocyte, comprising contacting a T lymphocyte with an effective amount of a regulatory reagent that is capable of altering the activity of an immunoreceptor tyrosine-based activation motif of a ζ chain of a T cell receptor.
A method to regulate actin polymerization in a T lymphocyte, comprising contacting a T lymphocyte with an effective amount of a regulatory reagent that alters the activity of an immunoreceptor tyrosine-based activation motif of an ε chain of a T cell receptor.
In a cellular system where a src-family tyrosine kinase is contacted with a T cell receptor chain selected from the group consisting of a ζ chain and an ε chain that is regulated by said src-family tyrosine kinase, the improvement comprising regulating actin polymerization by contacting a T lymphocyte with a reagent capable of binding to a protein selected from the group consisting of a third ITAM of a ζ chain, an ITAM of an ε chain and an SH2 domain.
A formulation capable of regulating actin polymerization in a T lymphocyte, said formulation comprising:
- (a) a regulatory reagent that alters the activity of a molecule selected from the group consisting of an immunoreceptor tyrosine-based activation motif of a ζ chain of a T cell receptor and an immunoreceptor tyrosine-based activation motif of a ε chain of a T cell receptor in a cell;
- and (b) a pharmaceutically acceptable carrier.
A kit to identify compounds capable of regulating actin polymerization in a T lymphocyte, said kit comprising:
- (a) a cell comprising a T cell receptor chain selected from the group consisting of a ζ chain, an ε chain, and actin monomers;
- and
- (b) a means for detecting the polymerization of said actin monomers.
Dependencies are inferred from the claim text.