Rh antibody
AssigneeThe Regents of the University of California
InventorsSherie L. Morrison, Ramon Montano
Life of this patent
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Abstract
Rh antibody hybrids for use in testing red blood cells for the presence of one or more Rh factors. The Rh hybrid antibody may also be used in therapeutic procedures which require the use of Rh antisera. The hybrid antibody includes an IgG anti-Rh antibody which has a polymeric tailpiece attached to the carboxy terminal end of each of the IgG antibody heavy chains. A hemagglutinin method is provided for Rh phenotyping in which agglutination of Rh-positive red blood cells is achieved in a one-step process involving addition of the hybrid Rh antisera to the red blood cells being tested.
Claims16
A hybrid anti-Rh antibody comprising:
- an IgG anti-Rh antibody which comprises two light chains and two heavy chains wherein said heavy chains each comprise an amino terminal end and a carboxy terminal end;
- and
- a polymeric tailpiece attached to the carboxy terminal end of each of said heavy chains.
In an IgG anti-Rh antibody which comprises a carboxy terminus, the improvement comprising attaching a polymeric tailpiece to the carboxy terminus of said antibody to provide an IgM/IgG anti-Rh hybrid antibody
A method for testing red blood cells to determine the presence of one or more Rh factors, said method comprising the steps of:
- (a) contacting red blood cells with an anti-sera which comprises an IgG anti-Rh hybrid antibody, said contact being for a sufficient time and at a sufficient temperature to cause agglutination of red blood cells which have one or more Rh factors present, and wherein said hybrid antibody comprises:
- an IgG anti-Rh antibody which comprises two light chains and two heavy chains wherein said heavy chains each comprise an amino terminal end and carboxy terminal end;
- and
- a polymeric tail piece attached to the carboxy terminal end of each of said heavy chains;
- and
- (b) determining whether said red blood cells agglutinate as a result of said contact with said IgG anti-Rh hybrid antibody.
Dependencies are inferred from the claim text.