Method for assaying creatine kinase isozyme activity and assay reagent
AssigneeInternational Reagents Corporation
InventorsYasushi Shirahase, Tadahiro Kajita, Tadashi Hoshino
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Abstract
A method for measuring the activity of creatine kinase MB (CK-MB) isozyme which is accurate, high in specificity, convenient, by inhibiting the activity of a mitochondria-localized creatine kinase (mCK) isozyme to avoid the influence of mCK and a measurement reagent therefor are provided. A method for measuring the activity of CK-MB isozyme comprising selectively measuring the enzymatic activity of CK isozymes by an immunological inhibition method, wherein after a treatment of selectively and immunologically eliminating the enzymatic actions of CK-M subunit and mCK is performed, the activity of remaining CK is measured.
Claims7
A method for measuring the activity of creatine kinase (CK) MB (CK-MB) isozyme comprising selectively measuring the enzymatic activity of CK isozymes by an immunological inhibition method, wherein after a treatment of selectively and immunologically eliminating the enzymatic actions of CK-M subunit and mCK is performed, the activity of remaining CK is measured.
A method for measuring the activity of mCK comprising measuring the activity of CK, acting an inhibitory antibody to mCK, and again performing the measurement.
Dependencies are inferred from the claim text.