Diagnosis of diseases associated with the immune system by determining cytosine methylation
OwnerAlexander Olek, Kurt Berlin +1inventors
InventorsAlexander Olek, Kurt Berlin, Christian Piepenbrock
Life of this patent
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Abstract
The present invention relates to chemically modified genomic sequences of genes associated with the immune system, to oligonucleotides and/or PNA-oligomers directed against the sequence, for the detection of the methylation status of genes, associated with the immune system as well as to a method for ascertaining genetic and/or epigentic parametres of genes, associated with the immune system.
Claims31
A nucleic acid comprising an at a least 18 bases-long sequence segment of the chemically pretreated DNA of genes associated with the immune system according to one of the Seq. ID No.1 through Seq. ID No.2420 and sequences complementary thereto.
A nucleic acid comprising an at a least 18 bases-long sequence segment of the chemically pretreated DNA of genes associated with the immune system according to one of the sequences of the genes A1BG (T80683), C4A (K02403), C4BPAL2 (X81360), CD1A (M27735), CD20 (L23418), CDR2 (M63256), CENPB (X05299), COL11A2 (U32169), CR1L (M31230), CYP2A (X06401), EBF (AA504812), ERCC2 (L47234), ESD (M13450), ETV4 (D12765), FCGR2C (M90737), FLG (M24355), FN1 (M10905), ITGA1 (X68742), ITGAD (U40274), KRT4 (X07695), KSR (U43586), LY9 (L42621), MEKK1 (U29671), MFAP4 (L38486), MMP18 (Y08622), MYCL1 (M19720), NOTCH1 (M73980), PDE7A (L12052), PIK3R1 (M61906), SLC9A1 (M81768), TCF3 (M31222), TCRA (M12959), TCRB (K02779), TCRG (M27331), TLR5 (U08888), TNFSF11 (AF013171), UBC (AB009010), ZNF121 (M99593), ZRK (L08961), ALPPL2 (NM—031313), AHSG (NM—001622), FCGR3A (NM—000569), FUT3 (NM—000149), IL1R2 (NM—004633), IL2RB (NM—000878), LHB (NM—000894), MDH2 (NM—005918), SLC11A2 (NM—000617), OMG (NM—002544), PIK3CA (NM—006218), TPM1 (NM—000366), TUB (NM—003320), ABAT (NM 000663), ACADL (NM—001608), ACO1 (NM—002197), ADAM10 (NM—001110), ADD1 (NM—014189), ADH4 (NM—000670), ADRA2C (NM—000683), AGA (NM—000027), AGTR2 (NM—000686), AKT1 (NM—005163), ALDH6 (NM—000693), AMPH (NM—001635), ANXA4 (NM—001153), APBA2 (NM—005503), APC (NM—000038), APOA2 (NM—001643), ARHGAP1 (NM—004308), ATOX1 (NM—004045), ATP2B2 (NM—001683), ATP4B (NM—000705), ATR (NM—001184), AUH (NM—001698), AXL (NM—001699), BCL2 (NM—000633), BENE (NM—005434), BID (NM—001196), BMI1 (NM—005180), BN51T (NM—001722), BUB1 (NM—004336), C1R (NM—001733), C4BPB (NM—000716), C5R1 (NM—001736), CASP3 (NM—004346), CASP7 (NM—001227), CBFB (NM—001755), CCR4 (NM—005508), CD151 (NM—004357), CD36L1 (NM—005505), CD4 (NM—000616), CD81 (NM—004356), CDH12 (NM—004061), CDW52 (NM—001803), CEL (NM—001807), CES1 (NM—001266), CGA (NM—000735), CHS1 (NM—000081), CLDN3 (NM—001306), CNK (NM—004073), CSF2RA (NM—006140), CTSK (NM—000396), CX3CR1 (NM—001337), CYBB (NM—000397), CYP11A (NM—000781), DCC (NM—005215), DFFB (NM—004402), DOCK1 (NM—001380), DPYD (NM—000110), ELAVL2 (NM—004432), ELAVL4 (NM—021952), EPB41 (NM—004437), EPHA3 (NM—005233), EPHX2 (NM—001979), EPS15 (NM—001981), ETV6 (NM—001987), F2 (NM—000506), F8A (NM—012151), FABP6 (NM—001445), FADD (NM—003824), FANCE (NM—021922), FCAR (NM—002000), FGA (NM—021871), FGB (NM—005141), FGFR3 (NM—000142), FGG (NM—000509), HFL3 (NM—005666), FOXO1A (NM—002015), ADAM2 (NM—001464), FUCA1 (NM—000147), FUT2 (NM—000511), FY (NM—002036), GABRA5 (NM—000810), GABRA6 (NM—000811), GAS (NM—000805), GAS6 (NM—000820), GBA (NM—000157), GFI1 (NM—005263), GH2 (NM—002059), GHR (NM—000163), GIF (NM—005142), GNAQ (NM—002072), GP9 (NM—000174), GPR15 (NM—005290), GPR30 (NM—001505), GRB14 (NM—004490), GRIK1 (NM—000830), GUCY2D (NM—000180), HADHA (NM—000182), NRG1 (NM—013964), HIVEP1 (NM—002114), HLALS (NM—001531), HLCS (NM—000411), HMX1 (NM—018942), HNRPD (NM—002138), HSA277165 (NM—018411), HRG (NM—000412), HSPG2 (NM—005529), HTN3 (NM—000200), HTR2A (NM—000621), HTR7 (NM—000872), IFNA1 (NM—024013), IL10RA (NM—001558), IL1A (NM—000575), IL1B (NM—000576), IL1R1 (NM—000877), IL3RA (NM—002183), IL9 (NM—000590), ILF1 (NM—004514), ILF2 (NM—004515), SCYB10 (NM—001565), INPP5D (NM—005541), ITGAX (NM—000887), ITGB1 (NM—002211), ITGB3 (NM—000212), ITGB5 (NM—002213), ITGB7 (NM—000889), ITK (NM—005546), KCNJ3 (NM—002239), KPNA1 (NM—002264), LECT2 (NM—002302), LEPR (NM—002303), LPA (NM—005577), KCNH2 (NM—000238), LSP1 (NM—002339), LTF (NM—002343), MAB21L1 (NM—005584), MAL (NM—002371), MASP1 (NM—001879), MCF2 (NM—005369), MAP3K3 (NM—002401), MMP16 (NM—022564), MMP17 (NM—016155), MMP23A (NM—004659), MMP7 (NM—002423), MYC (NM—002467), NAGA (NM—000262), NAT2 (NM—000015), NDUFS2 (NM—004550), NEB (NM—004543), NEU1 (NM—000434), NFATC4 (NM—004554), NFE2L2 (NM—006164), NFRKB (NM—006165), NGFB (NM—002506), NTF3 (NM—002527), NUMA1 (NM—006185), TNRC11 (NM—005120), SLC22A1L (NM—002555), DUSP2 (NM—004418), PAFAH2 (NM—000437), PAPPA (NM—002581), PCM1 (NM—006197), PCTK1 (NM—006201), PDE4A (NM—006202), PDE4B (NM—002600), PEX10 (NM—002617), SERPINB9 (NM—004155), PIGA (NM—002641), PLAGL1 (NM—002656), POU2AF1 (NM—006235), PRKG1 (NM—006258), MAPK10 (NM—002753), MAPK9 (NM—002752), PROP1 (NM—006261), PSD (NM—002779), PTK2B (NM—004103), PTN (NM—002825), PTPN13 (NM—006264), PTPN6 (NM—002831), PTPRD (NM—002839), PTPRG (NM—002841), QDPR (NM—000320), RAC3 (NM—005052), RELA (NM—021975), REQ (NM—006268), RMSA1 (NM—002932), RSN (NM—002956), S100A7 (NM—002963), S100A8 (NM—002964), IQGAP1 (NM—003870), SCN1B (NM—001037), SCN5A (NM—000335), SCNN1G (NM—001039), SCYA14 (NM—004166), SCYA7 (NM—006273), SDHC (NM—003001), SELPLG (NM—003006), SFTPA2 (NM—006926), SGSH (NM—000199), SHMT2 (NM—005412), MYH11 (NM—002474), SNRPN (NM—003097), SOAT1 (NM—003101), SORL1 (NM—003105), SPP1 (NM—000582), SSTR1 (NM—001049), STATI2 (NM—003877), STX1B (NM—003163), TCF8 (NM—030751), TCP1 (NM—030752), TF (NM—001063), TGFBI (NM—000358), TGFBR3 (NM—003243), TGM2 (NM—004613), TLR1 (NM—003263), TM4SF7 (NM—003271), TNFAIP6 (NM—007115), TNFRSF1A (NM—001065), TNFSF12 (NM—003809), TPH (NM—004179), TPI1 (NM—000365), TRAF2 (NM—021138), TRAF5 (NM—004619), TSTA3 (NM—003313), TTR (NM—000371), UBE1 (NM—003334), UBE2V2 (NM—003350), UMPK (NM—012474), UP (NM—003364), UPK1B (NM—006952), USP7 (NM—003470), VASP (NM—003370), VDR (NM—000376), NSEP1 (NM—004559), ZFP161 (NM—003409), AQP1 (NM—000385), BDKRB1 (NM—000710), F13A1 (NM—000129), and complementary sequences thereof.
A DNA- and/or PNA-array for analysing diseases associated with the methylation status of genes, comprising at least one nucleic acid according to one of the preceding claims.
Dependencies are inferred from the claim text.