DNA amplification and sequencing using DNA molecules generated by random fragmentation

A method of preparing a library of DNA molecules, comprising:

• obtaining a plurality of DNA molecules;
• randomly fragmenting at least one of the DNA molecules to produce DNA fragments;
• attaching a primer having a substantially known sequence to at least one end of a plurality of the DNA fragments to produce primer-linked fragments;
• and
• amplifying a plurality of the primer-linked fragments.

A method of generating a library of DNA templates, comprising:

• obtaining a plurality of DNA molecules;
• randomly fragmenting the plurality of DNA molecules to produce DNA fragments;
• attaching a first primer having substantially known sequence to at least one end of a plurality of the DNA fragments to produce primer-linked fragments;
• and
• amplifying a plurality of the primer-linked fragments, wherein the amplification utilizes:
• a second primer complementary to a known sequence in the DNA fragments;
• and
• a third primer complementary to the first primer.

A method of sequencing a plurality of DNA fragments concomitantly, comprising:

• obtaining a plurality of DNA molecules;
• randomly fragmenting the DNA molecules to generate a plurality of DNA fragments having overlapping sequences;
• attaching a first primer having a substantially known sequence to at least one end of the plurality of the DNA fragments to produce primer-linked fragments;
• and
• amplifying a plurality of the primer-linked fragments, wherein the amplification utilizes:
• a second primer complementary to a known sequence in the DNA fragments;
• and
• a third primer complementary to the first primer;
• and
• sequencing said plurality of DNA fragments using a fourth primer complementary to said known sequence in the DNA fragments.

A method of sequencing a consecutive overlapping series of nucleic acid sequences, comprising the steps of:

• obtaining a plurality of DNA molecules having overlapping sequences;
• concomitantly sequencing a first region in said plurality of DNA molecules using a primer complementary to a known sequence in said plurality of DNA molecules;
• and
• concomitantly sequencing a second region in said plurality of DNA molecules using a primer complementary to sequence determined from the sequencing of the first region,
• wherein the next consecutive sequencing of a region in the overlapping series of nucleic acid sequences is produced by initiating sequencing from the sequence obtained in a preceding overlapping sequencing product.

A method of preparing a DNA molecule having sequences that generate secondary structure in said molecule, comprising:

• obtaining the DNA molecule having said sequences;
• randomly fragmenting the DNA molecule to produce a plurality of DNA fragments, wherein the plurality of DNA fragments comprises DNA fragments having part or all of the sequences which generate the secondary structure;
• attaching a primer having substantially known sequence to at least one end of a plurality of the DNA fragments to produce primer-linked fragments;
• and
• amplifying a plurality of the primer-linked fragments.

A method of preparing a probe, comprising:

• obtaining at least one DNA molecule;
• randomly fragmenting the DNA molecule to produce DNA fragments;
• attaching a labeled primer having substantially known sequence to at least one end of a plurality of the DNA fragments to produce labeled primer-linked fragments;
• and
• amplifying a plurality of the primer-linked fragments.