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US 2003/0143598 A1Published application
Bioconjugate-nanoparticle probes
AssigneeNanosphere, Inc.
InventorsViswanadham Garimella, James J. Storhoff
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Abstract
The invention provides nanoparticle-bioconjugate probes that are useful for detecting target analytes such as nucleic acids. The probes of the invention are stable towards heat and resistant to displacement by thiol containing compounds such as DTT (dithiothreitol).
Claims99
A nanoparticle-probe comprising a bioconjugate coupled to a nanoparticle, wherein the bioconjugate is:
- 13
- wherein
- n is 2-100;
- m is 0-100;
- X is a nucleotide or modified nucleotide;
- Z is a nucleotide, modified oligonucleotide, or polyanion;
- Q is a recognition group;
- R is linear or branced C1-C8 alkyl;
- W is a steroid;
- and
- each L is a linker formed by the coupling of two moieties selected from the group consisting of COOH, NH2, CHO, F, Cl, Br, I, NCO, NCS, allyl, vinyl, and CH3CO2−, or L is —C(═NH2Cl)(CH2)3−,
- wherein the bioconjugate is coupled to the nanoparticle through the sulfur groups.
9 more elements
44 The method of claim 41 wherein the alkyl thiol amine is SH(CH2)nNH2.
Dependencies are inferred from the claim text.