Genetic hypermutability of plants for gene discovery and diagnosis

A method for generating herbicide susceptible weeds from herbicide resistant weeds comprising:

• (a) mutagenizing said resistant weeds, thereby creating mutant parental weeds;
• (b) testing progeny of said mutant parental weeds for susceptibility to said herbicide;
• and
• (c) selecting said mutant parental weeds producing herbicide susceptible progeny.

A method for generating herbicide resistant weeds from herbicide susceptible weeds comprising:

• (a) mutagenizing said susceptible weeds, thereby creating mutant parental weeds;
• (b) testing progeny of said mutant parental weeds for resistance to said herbicide;
• and
• (c) selecting said mutant parental weeds producing herbicide resistant progeny.

A method for identifying a mutant gene conferring herbicide resistance comprising

• (a) comparing the genome of a naturally occurring herbicide resistant plant to the genome of an herbicide susceptible plant;
• (b) determining genetic differences between said herbicide resistant plant to the herbicide susceptible plant;
• and
• (c) sequencing a region of DNA comprising said genetic difference.

A method for identifying a mutant gene conferring herbicide susceptibility comprising

• (a) introducing into an herbicide resistant weed gene fragments from an herbicide susceptible weed, thereby creating a transfected herbicide resistant strain;
• (b) screening progeny of said transfected herbicide resistant strain for herbicide susceptibility;
• and
• (c) sequencing said gene fragment to identify an herbicide susceptibility gene.

A method for identifying a mutant gene conferring herbicide susceptibility comprising

• (a) crossing an herbicide resistant weed with an herbicide susceptible weed, thereby creating a crossed strain;
• (b) screening progeny for herbicide susceptibility;
• and
• (c) performing genetic analysis on said crossed strain producing herbicide susceptible progeny to identify an herbicide susceptibility gene.

A method for identifying a mutant gene conferring herbicide resistance comprising:

• (a) crossing an herbicide resistant weed with an herbicide susceptible weed, thereby creating a crossed strain;
• (b) screening progeny for herbicide resistance;
• and
• (c) performing genetic analysis on said crossed strain producing herbicide resistant progeny to identify an herbicide resistance gene.

A method for identifying a mutant gene conferring herbicide resistance comprising

• (a) mutagenizing an herbicide susceptible weed, thereby creating mutant parental weeds;
• (b) testing progeny of said mutant parental weeds for resistance to said herbicide;
• and
• (c) comparing the genome of a naturally occurring herbicide resistant plant to the genome of an herbicide susceptible plant;
• (d) determining genetic differences between said herbicide resistant plant to the herbicide susceptible plant;
• and
• (e) sequencing a region of DNA comprising said genetic difference.

A method for identifying a mutant gene conferring herbicide resistance comprising

• (a) mutagenizing an herbicide resistant weed, thereby creating mutant parental weeds;
• (b) testing progeny of said mutant parental weeds for susceptibility to said herbicide;
• and
• (c) comparing the genome of a naturally occurring herbicide resistant plant to the genome of an herbicide susceptible plant;
• (d) determining genetic differences between said herbicide resistant plant to the herbicide susceptible plant;
• and
• (e) sequencing a region of DNA comprising said genetic difference.

A method for generating genetically stable glyphosate susceptible weeds derived from glyphosate resistant parental weeds comprising:

• (a) contacting said glyphosate susceptible weed with an inhibitor of mismatch repair, thereby forming a hypermutable parental weed;
• (b) testing progeny of said hypermutable parental weed that are glyphosate susceptible;
• (c) selecting hypermutable parental strains producing glyphosate susceptible progeny;
• (d) removing said inhibitor of mismatch repair from said hypermutable parental weed, thereby making said hypermutable parental weed genetically stable;
• and
• (e) obtaining progeny from genetically stable parental weed.

An oligonucleotide primer that anneals under PCR conditions to a polymorphic marker in genomic DNA or cDNA of a plant, wherein the nucleotide sequence of said polymorphic marker is selected from the group consisting of SEQ ID NO:

• 16, SEQ ID NO:
• 60, SEQ ID NO:
• 61, SEQ ID NO:
• 126, SEQ ID NO:
• 127, SEQ ID NO:
• 128, SEQ ID NO:
• 77, SEQ ID NO:
• 78, SEQ ID NO:
• 79, SEQ ID NO:
• 80, SEQ ID NO:
• 81, SEQ ID NO:
• 82, SEQ ID NO:
• 83, SEQ ID NO:
• 84, SEQ ID NO:
• 85, SEQ ID NO:
• 86, SEQ ID NO:
• 87, SEQ ID NO:
• 88, SEQ ID NO:
• 89, SEQ ID NO:
• 90, SEQ ID NO:
• 91, SEQ ID NO:
• 92, SEQ ID NO:
• 93, SEQ ID NO:
• 94, SEQ ID NO:
• 95, SEQ ID NO:
• 96, SEQ ID NO:
• 97, SEQ ID NO:
• 98, SEQ ID NO:
• 99, SEQ ID NO:
• 100, SEQ ID NO:
• 101, SEQ ID NO:
• 102, SEQ ID NO:
• 103, SEQ ID NO:
• 104, SEQ ID NO:
• 105, SEQ ID NO:
• 106, SEQ ID NO:
• 107, SEQ ID NO:
• 108, SEQ ID NO:
• 109, SEQ ID NO:
• 110, and SEQ ID NO:
• 111, and wherein said oligonucleotide primer is at least 15 nucleotides in length, and comprising at least 85% identity to a region of said polymorphic marker.

A method of identifying a therapeutic compound to increase a resistance to herbicides in a plant comprising:

• (a) introducing a gene conferring herbicide susceptibility into a plant;
• (b) isolating purified protein from said plant;
• (c) contacting said protein with a panel of candidate compounds;
• (d) selecting compounds that bind to said protein;
• and
• (e) screening for the ability of a selected compound to interfere with herbicide susceptibility;
• thereby identifying a therapeutic compound.