Solid-phase reactions
AssigneeNorthwestern University
InventorsPriscilla Wilkins Stevens, David Kelso, Victor Lyamichev, Jeff Hall, Bruce P. Neri, Robert W. Kwiatkowski JR., Lloyd M. Smith
Life of this patent
Estimating the term…
Abstract
The present invention relates to systems, compositions, and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for attaching nucleic acids to solid supports and modifying nucleic acids. For example, in some embodiments, the 5′ nuclease activity of a cleavage agent is used to cleave a cleavage structure formed on the solid support, the occurrence of the cleavage event indicating the presence of specific nucleic acid sequences.
Claims22
A method for detecting polymorphisms in unamplified DNA, comprising:
- a. providing:
- i. unamplified DNA;
- ii. reagents for conducting an invasive cleavage reaction, wherein at least one of said reagents is attached to a solid surface via a spacer molecule;
- b. treating said unamplified DNA with said reagents under conditions such that the presence or absence of a polymorphism in said genomic DNA is identified.
A kit for detecting polymorphisms in unamplified DNA, comprising:
- reagents for conducting an invasive cleavage reaction, wherein at least one of said reagents is attached to a microsphere solid surface via a spacer molecule.
Dependencies are inferred from the claim text.